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Purdue/25 September 2008
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| + | Performed ligation reaction with LacZ and SOS: | ||
| + | *Mixture: | ||
| + | **1uL T4 ligase | ||
| + | **2uL T4 buffer | ||
| + | **5uL LacZ DNA | ||
| + | **12uL SOS DNA | ||
| + | *Let sit for XXXX minutes at room temperature | ||
| + | *Heat inactivated enzyme for 10 min at 65C | ||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Revision as of 15:56, 25 September 2008
Click Here to return to the notebook.
Performed ligation reaction with LacZ and SOS:
- Mixture:
- 1uL T4 ligase
- 2uL T4 buffer
- 5uL LacZ DNA
- 12uL SOS DNA
- Let sit for XXXX minutes at room temperature
- Heat inactivated enzyme for 10 min at 65C
Edited by Janie Stine
