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Purdue/9 September 2008
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| + | *Jess re-did miniprep/digestion of LacZ and SOS (as before) | ||
| + | *Jess ran on ethidium bromide gel yesterday (pictures in notebook) | ||
| + | *Got a LacZ fragment band at 5000 bp (yay!--is the length of part + most of plasmid) | ||
| + | *LacZ fragments are stored for later purification | ||
| + | *Got an SOS fragment at 2000 bp (boo!--is the length of the plasmid minus the part) | ||
| + | **The part ran off the gel b/c is only 192 bp long | ||
| + | *PLAN: redo SOS miniprep, etc. but run shorter gel so can get the SOS part before it runs off | ||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Latest revision as of 14:52, 9 September 2008
Click Here to return to the notebook.
- Jess re-did miniprep/digestion of LacZ and SOS (as before)
- Jess ran on ethidium bromide gel yesterday (pictures in notebook)
- Got a LacZ fragment band at 5000 bp (yay!--is the length of part + most of plasmid)
- LacZ fragments are stored for later purification
- Got an SOS fragment at 2000 bp (boo!--is the length of the plasmid minus the part)
- The part ran off the gel b/c is only 192 bp long
- PLAN: redo SOS miniprep, etc. but run shorter gel so can get the SOS part before it runs off
Edited by Janie Stine
