"
Rensselaer/22 August 2008
From 2008.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000]] on pSB1A2. | To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000]] on pSB1A2. | ||
| - | The tentative schedule is that we will transform ''E. coli'' cells with the plasmids that we want to amplify Wednesday evening or Thursday morning | + | The tentative schedule is that we will transform ''E. coli'' cells with the plasmids that we want to amplify Wednesday evening or Thursday morning, and will then do a restriction digest on the DNA to test the restriction sites on the plasmids ( SpeI [[http://openwetware.org/wiki/SpeI]] and XbaI [[http://openwetware.org/wiki/XbaI]] ). After the restriction digest, the size of the fragments should be 1.05 kb and 2.90 kb. We will also run a control without a digest to make sure that the plasmids remain circular. |
Revision as of 20:06, 22 July 2008
9:00 AM Pip, James, Dimitre
To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[1]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[2]] on pSB1A2.
The tentative schedule is that we will transform E. coli cells with the plasmids that we want to amplify Wednesday evening or Thursday morning, and will then do a restriction digest on the DNA to test the restriction sites on the plasmids ( SpeI [[3]] and XbaI [[4]] ). After the restriction digest, the size of the fragments should be 1.05 kb and 2.90 kb. We will also run a control without a digest to make sure that the plasmids remain circular.
