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User contributions
From 2008.igem.org
- 23:12, 16 September 2008 (diff | hist) N Rensselaer/16 September 2008 (New page: '''Setting up primers for PCR''' Added Nuclease free water to dried primers to obtain a 100 micromolar concentration stock. e.g. 30.5 nm(1/350 uL) = 100 pm/uL primers for PCR are to a...) (top)
- 22:28, 15 September 2008 (diff | hist) N Rensselaer/15 September 2008 (New page: '''Ligation of Fe promoter and ZntA promoter to deP mRFP vector.''' Ratio is 3 insert to 1 vector '''approximate sizes''' 2kb vector 200 bp ZntA 500 bp Fe need 200 ng total: 3x conc...) (top)
- 05:59, 15 September 2008 (diff | hist) N Rensselaer/11 September 2008 (New page: '''Purification:''' 1. Added X uL of Membrane Binding solution to X uL Fe promotor and X uL of Membrane Binding Solution to X uL mRFP insert. Pipetted to mix and incubated for 1 minute...) (top)
- 05:46, 15 September 2008 (diff | hist) N Rensselaer/12 September 2008 (New page: Goal: to purify the ZntA promoter and the dephosphorylated mRFP insert, and to quantify the amount of the resulting purifications as well as phosphorylated mRFP and the Fe promoter. '''Pu...) (top)
- 20:55, 8 September 2008 (diff | hist) Rensselaer/8 September 2008 (top)
- 20:49, 8 September 2008 (diff | hist) N Rensselaer/8 September 2008 (New page: '''DNA purification digest''' Fe(13ug) Plasmid(11ug) DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 ...)
- 19:22, 2 August 2008 (diff | hist) Rensselaer/30 July 2008
- 20:16, 22 July 2008 (diff | hist) N Rensselaer/22 July 2008 (New page: '''9:00 AM Pip, James, Dimitre''' To test the different promoters for the project, we decided on using the plasmid pSB1A2 http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 wh...) (top)
- 20:16, 22 July 2008 (diff | hist) Rensselaer/22 August 2008 (Removing all content from page) (top)
- 20:06, 22 July 2008 (diff | hist) Rensselaer/22 August 2008
- 19:55, 22 July 2008 (diff | hist) Team:Rensselaer (top)
- 19:51, 22 July 2008 (diff | hist) Rensselaer/22 August 2008
- 19:40, 22 July 2008 (diff | hist) N Rensselaer/22 August 2008 (New page: '''9:00 AM Pip, James, Dimitre''' To test the different promoters for the project, we decided on using the plasmid pSB1A2 http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 wh...)
- 19:02, 15 July 2008 (diff | hist) Team:Rensselaer
- 18:38, 15 July 2008 (diff | hist) Team:Rensselaer
- 18:29, 15 July 2008 (diff | hist) Team:Rensselaer
- 09:05, 3 July 2008 (diff | hist) N Rensselaer/2 July 2008 (New page: '''Meeting synopsis for July 2nd.''' Lab work will commence two weeks from now. Our task for next week is to plan the steps that need to take place in order to get our project off the whi...) (top)
- 08:51, 3 July 2008 (diff | hist) Team:Rensselaer/Notebook (→Notebook)
- 17:47, 1 July 2008 (diff | hist) Team:Rensselaer/Project
