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Rensselaer/22 August 2008
From 2008.igem.org
9:00 AM Pip, James, Dimitre
To test the different promoters for the project, we decided on using the plasmid pSB1A2 http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000 on pSB1A2.
The tentative schedule is that we will transform E. coli cells with the plasmids that we want to amplify Wednesday evening or Thursday morning, and will then do a restriction digest on the DNA to test the restriction sites on the plasmids ( SpeI http://openwetware.org/wiki/SpeI and XbaI http://openwetware.org/wiki/XbaI ). After the restriction digest, the size of the fragments should be 1.05 kb and 2.90 kb. We will also run a control without a digest to make sure that the plasmids remain circular.
