User contributions
From 2008.igem.org
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- 12:48, 29 October 2008 (diff | hist) N Melbourne/17 July 2008 (New page: ==Done today:== * Re-transformed P22 6E (RL), P13 4F, 8A (FBL)--plated on Kan+IPTG * Double transformation of pLPCB & CPH8 into RU1012 cells--to be plated tomorrow on Amp+Kan+Chlor+IPTG+X-...) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/15 July 2008 (New page: ==Done today:== * Miniprepped PO 3F, 3E, 4C, 4G * Set up cultures P1 7E, P13 9G) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/14 July 2008 (New page: ==Done today:== * Re-transformed: ** P13 4F, 8A (FBL) ** P22 6E, P13 9G (RL) ** P1 7E (RS) ''Result:'' Only P13 9G and P1 7E grew. * Made Kan plates) (top)
- 12:47, 29 October 2008 (diff | hist) N Melbourne/11 July 2008 (New page: ==Done today:== * Miniprepped P13 9H, P6 3D, P2 9A, P13 5E, P13 5C) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/9 July 2008 (New page: ==Done today:== * Re-transformed P13 9H (RL), P6 3D (RS) * Suspended and transformed P2 9A (RS) Used slightly modified protocol. All plates showed growth.) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/8 July 2008 (New page: ==Done today:== * Retransformed : P13 4F, 8A (FBL) P13 9G, P13 9H, P22 6E (RL) Also set up a positive control (pET 30-8)--Kanamycin resistant vector * Suspended and transformed P6 3...) (top)
- 12:45, 29 October 2008 (diff | hist) N Melbourne/3 July 2008 (New page: ==Done today:== * Miniprepped: ** P0 C4, E3, F3, G4 (FBL) ** P3 8C, P1 12F (RS) * Made glycerol stocks * Transformed: ** P22 8C, 6E ** P16 5F ** P13 5E, 9G ** P2 5E ** P0 2E * Punched:...) (top)
- 12:44, 29 October 2008 (diff | hist) N Melbourne/1 July 2008 (New page: ==Done today:== * Suspended from Kit {| border = "0.5" |- ! Part ! Plate ! Well |- | M30109 | 1022 | 8C |- | I15010 | 1022 | 6E |- | I15008 | 1013 | 9G |- | I15009 | 1013 | 5E |- | R00...) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/30 June 2008 (New page: ==Done today:== * Made competent DH5alpha cells.) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/1 June 2008 (New page: ==Done today:== * Suspension and transformation (of DH5alpha cells) with P1003 8C (J36846 pTet mRFP)...for ribolock as reporter P1002 8A (I13522 pTet GFP) P1002 3H (I13600 pTet CFP) ...) (top)
- 12:41, 29 October 2008 (diff | hist) N Melbourne/20 May 2008 (New page: ==Done today:== *Preparation of pL-pCB and pCph8 for sequencing ** 100μM primer stock stored in red light box in -20°C freezer *O/N culture of pL-pCB and pCph8 for miniprep tomorrow ...) (top)
- 12:40, 29 October 2008 (diff | hist) N Melbourne/13 May 2008 (New page: ==Done today:== ''Glycerol stocks'' made from overnight cultures (see yesterday for details) *800μL culture *600μL 100% glycerol Stored in -...) (top)
- 12:39, 29 October 2008 (diff | hist) N Melbourne/12 May 2008 (New page: ==Done today:== Transformations and overnight culture for glycerol stocks- Three cultures: *pCph8 in RU1012 cells *pL-pCB in RU1012 cells *pCph8/pL-pCB in RU1012 cells) (top)
- 12:38, 29 October 2008 (diff | hist) N Melbourne/5 May 2008 (New page: ==Done today:== Transformation for glycerol stocks: *RU1012 cells *1 μL DNA *Three transformations: **pCph8 **pL-PCB **pCph8/pL-PCB *Standard transformation protocol) (top)
- 12:37, 29 October 2008 (diff | hist) N Melbourne/16 April 2008 (New page: ==Done today:== Red light test (cont): Unfortunately both plates turned blue. This was most likely because the control was not in the light all the time. The pPL-PCB plasmid was not indu...) (top)
- 12:36, 29 October 2008 (diff | hist) N Melbourne/15 April 2008 (New page: ==Done today:== Red light test *Double transformation that Alisa did was used as folows: *2 plates (Amp/Kan/Chlor) had Xgal added (20 mg/mL) by disolving 1.6 mg of Xgal in 80 μL of DMSO ...) (top)
- 12:24, 29 October 2008 (diff | hist) N Melbourne/11 April 2008 (New page: ==Done today:== * Ru1012 cells are competent (less effient than DH5α)) (top)
- 12:24, 29 October 2008 (diff | hist) N Melbourne/10 April 2008 (New page: ==Done today:== RU1012 cells were tested for competency *1 μL of P48J test plamid used *30 min on ice *1 min heat shock at 42°C *Alisa plated samples on Amp plates) (top)
- 12:22, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 12:19, 29 October 2008 (diff | hist) N Melbourne/4 April 2008 (New page: ==Done today== *Mini-Prep of two CPH8 cultures and two pL-pCB cultures *Stored in iGEM freezer) (top)
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