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Team:Purdue/Tools and References
#Web of Science page for "Binding of Photolyase of ''E. coli'' to UV-Damaged DNA" [http://www2.lib.purdue.edu:2149/full_record.do?produc #Sequences of the ''E. coli'' Photolyase gene and protein: [http://www2.lib.purdue.edu:2479/cgi/reprin

6 KB (864 words) - 18:00, 10 July 2008
Team:Caltech
...mally present in the large intestine. Because of its use in research, ''E. coli'' is the most well characterized bacteria to date. Another bacteria, ''Bact ...l bacterial species can cause disease in humans by infecting the gut. ''E. coli'' is commonly associated with food poisoning. ''Salmonela enterica'' is res

8 KB (1,094 words) - 00:53, 30 October 2008
Team:Utah State/Project
recombinant ''E. coli'' and in ''C. necator''. In order to develop an ...tributed to a cost discrepancy of $1.6/kg (Lee SY, 1998). Recombinant ''E. coli'' cells harboring PHB production genes from ''C. necator'' were used in thi

16 KB (2,351 words) - 03:56, 30 October 2008
Team:University of Lethbridge/Project
...we intend to have our riboswitch express the motility protein cheZ in ''E. coli'', thus directing the bacterium towards higher concentrations of our target ...hich responds to this target, a gene involved in motility in ''Escherichia coli'' (''CheZ'') will be switched to an 'on' state (Topp and Gallivan, 2007). '

13 KB (1,787 words) - 04:30, 30 October 2008
Team:TUDelft/Recommendations
...ing our envisioned color pathway by PCR-ing three of the genes out of ''E. coli'', which have been sent to the registry and to a sequencing company. ...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin

6 KB (985 words) - 12:47, 29 October 2008
Team:University of Alberta/Plastic Project:The University of Alberta
We aim to move the human estrogen receptor (hER) into E. coli, which will activate a reporter system in the presence of BPA. When BPA is ... periplasmic and cytoplasmic protein–protein interactions in Escherichia coli: minimal requirements for specific DNA binding and transcriptional activati

14 KB (2,168 words) - 05:32, 30 October 2008
Team:Caltech/Project/Population Variation
... front of LacZ, and then chromosomally integrating the construct into ''E. coli'' so that there is only one copy of the construct in a cell. Our current wo ..., Marcotte EM, and Voigt CA. '''Synthetic biology: engineering Escherichia coli to see light'''. Nature 2005 Nov 24; 438(7067) 441-2.

20 KB (3,252 words) - 18:01, 21 October 2008
Team:TUDelft/Future Work
...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin ...be tested for individual activity, which has not yet been done on the ''E. coli'' genes we provided to the registry. If enzyme activity has been confirmed,

4 KB (599 words) - 22:55, 29 October 2008
Team:Calgary Software/E.Coli CAL516
... scanning electron image of a single E. Coli CAL516 surrounded by other E. Coli bacteria]] ...ythology has been created about confirmed and unconfirmed properties of E. Coli Calgarius. Much of the folklore that surrounds actor [[Team:Calgary_Wetware

978 B (149 words) - 18:03, 25 October 2008
Team:IIT Madras/Project
...ressed sigma-24, sigma-28, sigma-32 and sigma-38 promoters for Escherichia coli. ...truct, and validate a library of sigma-dependent promoters for Escherichia coli, with the following design specifications:

7 KB (1,125 words) - 14:42, 27 October 2008
Team:TUDelft/Color design
...mes from the registry. Of the eight enzymes we will isolate three are ''E. coli'' enzymes (atoB, idi and ispA), while the other five are ''S. cerevisiae'' To PCR the genes out of the ''E. coli'' genome, we've designed primers using the invitrogen website. These primer

5 KB (764 words) - 17:36, 29 October 2008
Team:Slovenia/Notebook/Methods
... DH5α strain was used. After DNA sequences verification E. coli BL21(DE3)pLysS were transformed with specific constructs for the overex ...ogenization Kit (Novagen) to integrate λDE3 prophage into the E. coli host cell chromosome. λDE3 is a recombinant phage carrying a clo

48 KB (6,976 words) - 04:42, 30 October 2008
Team:University of Ottawa/Project
...s are often expressed in simple microorganisms such as bacteria (e.g. ''E. coli'') or yeast. The two most used yeast species are ''Saccharomyces cerevisiae ...urrently limited when compared to the spectrum of parts developed for ''E. coli'', we thought that this would be a novel yeast-based system to expand upon.

22 KB (3,281 words) - 00:50, 30 October 2008
Team:ETH Zurich/Wetlab/Genome Reduction
...tion we discuss about how to randomly delete chromosomal fragments of ''E. coli'' in order to reduce the physical amount of genomic DNA. ...neArt and is supposed to be integrated into the genome of a wild-type ''E. coli'' strain (MG1655). We are planning to use the lambda red recombination syst

12 KB (1,777 words) - 04:34, 30 October 2008
Team:NTU-Singapore/Wetlab/Materials and Equipment
A colony of E. coli W3110 was grown overnight in LB for 16 hours at 37°C . The overnight cultu ...used in all the experiments were LuxS mutants derived from the wild type E coli strain W3110. By knocking out LuxS gene, the cells were unable to synthesiz

6 KB (869 words) - 05:47, 27 October 2008
Team:Bologna/Biosafety
...ral kinds of bacteria and viruses [including canine hepatitis, Escherichia coli, varicella (chicken pox)], as well as some cell cultures and non-infectious ==Genetically modified ''E. coli''==

29 KB (4,412 words) - 02:19, 30 October 2008
Team:Heidelberg/Project/Sensing
...rjik, 2004). For a schematic presentation of the chemotaxis signaling in E.coli see figure 1. ...ain objective of our project is to engineer a so called killer strain of E.coli which would be able to swim by chemotaxis towards a “prey” strain whic

35 KB (5,074 words) - 22:59, 29 October 2008
Team:NTU-Singapore/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

5 KB (768 words) - 14:55, 28 October 2008
Team:KULeuven/Model/Filter
...s of the ribokey/lock and the difference in transcription rates between E. coli's RNA polymerase II and phage T7's polymerase. ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

15 KB (2,344 words) - 23:12, 29 October 2008
Team:NTU-Singapore/Team/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

4 KB (577 words) - 15:07, 20 October 2008