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Team:Alberta NINT/Protocols
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Coming Soon! | Coming Soon! | ||
| + | '''Overnights''' | ||
| + | Inoculating LB culture tubes: | ||
| + | Add 5ul Amp50 or Amp100 to 200ml LB broth in tube. | ||
| + | Using sterile inoculating loop, touch an isolated colony. | ||
| + | Swirl loop through LB + amp broth. | ||
| + | Incubate overnight at 37 C. | ||
Revision as of 17:31, 17 June 2008
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Lab Protocols
Coming Soon! Overnights Inoculating LB culture tubes:
Add 5ul Amp50 or Amp100 to 200ml LB broth in tube. Using sterile inoculating loop, touch an isolated colony. Swirl loop through LB + amp broth. Incubate overnight at 37 C.
