Team:BrownTwo/Implementation/yeast

sassy indeed

• eukaryote- closer to humans & important plants
• is itself important in industry
• inherent cooperativity of transcription
• genomic integration
  • stability
  • copy number control

Understanding our chassis

Yeast are a well-characterized yet relatively simple eukaryotic model. This is important given that we desired our results to have eventual application to mammalian models in which abnormal gene regulation can lead to disease conditions.

One important consideration to keep in mind while switching from the E. coli standard to yeast is that the signals for transcription and translation differ. In E. coli, sigma factors dictate the act of transcription. There are few distinct sigma factors present and

Another key point in the comparison between prokaryotic and eukaryotic systems is that, due to the extensive compartmentalization seen in eukaryotic cells, transcription and translation occur within different locations of the cell. The two processes are also separated by, during which additional modifications are made to the precursor mRNA before it leaves the nucleus.

Kozak sequence 5' cap of mRNA required for initiation of translation in eukaryotes This is analogous to the RBS region in prokaryotes