Team:Cambridge

From 2008.igem.org

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==MIGRATION: Note to Cambridge iGEMers==
==MIGRATION: Note to Cambridge iGEMers==
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*[[Team:Cambridge/Bacillus]]-> Linda
*[[Team:Cambridge/Bacillus]]-> Linda
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This year, the Cambridge iGEM team is working towards creating an integrated Bacterial Recombinant Artificial Intelligence Network (iBRAIN). Our concept is to model eukaryotic neural behaviour using populations of bacteria. We are looking at two main aspects of this concept: self-organisation using Turing pattern formation, and synaptic signal transduction using voltage output glutamate detection
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[[iGEM:Cambridge/2008/Concept |<font style="color:#cccccc">(read more...)]]
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!align="center"|[[Team:Cambridge|Home]]
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!align="center"|[[Team:Cambridge/Team|The Team]]
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    <td width="370"><a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation" class="noborder"><img src="http://openwetware.org/images/9/9d/Signalling_button.gif" alt="Signalling" width="363" height="236"></a></td>
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!align="center"|[[Team:Cambridge/Project|The Project]]
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    <td width="200"><a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Bacillus"><img src="http://openwetware.org/images/8/8e/Bacillus_button.gif" alt="Bacillus" width="363" height="236"></a></td>
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!align="center"|[[Team:Cambridge/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cambridge/Modeling|Modeling]]
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!align="center"|[[Team:Cambridge/Notebook|Notebook]]
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  <td><a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Voltage"><img src="http://openwetware.org/images/7/74/Voltage_button.gif" alt="Voltage" width="363" height="236"></a></td>
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  <td><a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Modelling"><img src="http://openwetware.org/images/9/91/Modelling_button.gif" alt="Modelling" width="363" height="236"></a></td>
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----
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[[IGEM:Cambridge/2008/PresentationOutline | <font face="verdana" style="color:#ffffff"> '''Poster & Presentation Outline''' </font>]]
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|[[Image:Cambridge_2008 Logo.gif|centre|400px]]
 
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= '''[http://openwetware.org/wiki/IGEM:Cambridge/2008 For updated information, please visit our main Wiki on OpenWetWare]''' =
 
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= Project Summaries =  
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===Sponsors===
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'''For the 2008 iGEM competition, Cambridge is working on three different projects.'''
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== 1. Turing Patterns ==
 
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We are planning to implement a simple two-component Reaction-Diffusion system in the gram-positive model organism Bacillus subtilis. In 1952, Alan Turing famously described this system and suggested it as the basis for self-organization and pattern formation in biological systems. The simplest of these patterns, which we are planning to model in bacteria, mimic the spots and stripes seen on animal coats.
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    <td width="200"><a href="http://www.labtech.co.uk/" class="noborder"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/labtech.jpg" alt="labtech" width="131" height="17"></a></td>
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    <td width="200"><a href="http://www.clontech-europe.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/clontech.jpg" alt="Clontech" width="112" height="34"></a></td>
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  <td width ="200"><a href="http://www.expressys.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/expressys.jpg" alt="expressys" width="112" height="70"></a></td>
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  <td><a href="http://www.invitrogen.com/site/us/en/home.html"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/invirtogen.jpg" alt="invitrogen" width="160" height="25"></a></td>
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  <td><a href="http://www.medical-solutions.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/geneservice.jpg" alt="geneservice" width="140" height="26"></a></td>
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    <td><a href="http://www.bioscience.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/cambridgebioscience.jpg" alt="cambridge bioscience" width="157" height="42"></a></td>
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    <td><a href="http://www.zymoresearch.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/zymoresearch.jpg" alt="zymo research" width="135" height="45"></a></td>
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    <td><a href="http://www.vwr.com/index.htm"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/vwr.jpg" alt="VWR" width="140" height="47"></a></td>
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  <td><a href="http://www.microzone.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/microzone.gif" alt="Microzone" width="131" height="44"></a></td>
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    <td><a href="http://www.finnzymes.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/finnzymes.jpg" alt="Finnzymes" width="131" height="43"></a></td>
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      <td><a href="http://www.fisher.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/fisherscientific.jpg" alt="Fisher Scientific" width="132" height="33" /></a></td>
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    <td><a href="http://www.dna20.com/"><img src="http://openwetware.org/images/8/88/Dna20_logo.jpg" alt="DNA 2.0" width="132" height="66" /></a></td>
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<center>http://www.uni-muenster.de/Physik.AP/Purwins/RD/2kgl.gif</center>
 
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(A) The model consists of two diffusible signals secreted by every cell. The activator, which is controlled by a stochastic bistable switch, turns on itself and its own inhibitor. (B) A field of cells can be stably patterned into two different zones, so long as the inhibitor diffuses faster than the activator. The activator and inhibitor are synthesized in the source at the center, and turned off by accumulation of the inhibitor in the periphery.
 
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http://parts.mit.edu/igem07/images/3/32/Cambridge_Agr_operon_and_biochemical_pathways.png
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[[iGEM:Cambridge/2008/Help | Help]]
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We plan to use two well-characterized bacterial communication systems to generate this behavior.  The agr peptide signalling system from S. aureus will serve as our activatory signal (pictured), while the lux system from V. fischeri will serve as our inhibitor. Bacillus subtilis serves as an excellent chassis for this project because of the ease with which chromosomal integration can be performed. This project will focus on a tight integration of modeling and experiment; we will test different promoter strengths and other variables, feed these system parameters into our multi-cell models, and then use those models to tweak the regulatory machinery that will control signal production.
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== 2. Voltage Output ==
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The aim of this project is to work towards an interface between biological and electric systems. We hope to do this by measuring a voltage change due to the presence of a certain substance.
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Today = new Date();
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In the first instance, this substance will be glutamate, as it acts as a ligand for a prokaryotic gated potassium channel.  
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Jamboree = new Date("November 8, 2008");
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Our idea is to sequester K+ inside E.coli cells by using leak channel knock-out mutants, and over-expressing K+ influx pumps. Then, when glutamate is present it will open K+ channels, allowing an efflux of potassium and causing a small but measureable change in voltage in the medium.
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msInADay = 1000 * 60 * 60 * 24;
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document.write("There are " + display +" days left until the Jamboree!");
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== 3. Magnetic Bacteria ==
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We are investigating the formation of magnetosomes (membrane bound magnetite particles) in magnetotactic bacteria. This process is believed to take place in the following steps:
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i) production of invaginations along the inner membrane
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ii) uptake of iron into these invaginations
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iii) biomineralisation of the iron into magnetite crystals of a specific size and shape
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iv) axial alignment of the magnetosomes
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This mechanism gives the bacteria the ability to align itself like a compass needle along geomagnetic field lines.
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We are attempting to engineer the uptake of soluble iron into membrane invaginations in E.coli, and stimulate formation of magnetite using genes from Magnetospirillum.
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Revision as of 16:21, 28 October 2008

Home   Voltage   Signalling   Bacillus   Modelling   Protocols   People  

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MIGRATION: Note to Cambridge iGEMers

We are manually transferring all wiki text and images from OWW to this page. Please check the email you were sent for your particular subsection. Please transfer images as well as text. Transferring images requires downloading them to your computer and re-uploading them to this website.

Once all the data is here, we will begin formatting everything properly with CSS and HTML. Here are temporary links to the subsections:

P.S. I'm saving the html scripts I've changed in case anyone needs them back! - Kath

---To be deleted after completion of moving stuff into Wiki End!---


This year, the Cambridge iGEM team is working towards creating an integrated Bacterial Recombinant Artificial Intelligence Network (iBRAIN). Our concept is to model eukaryotic neural behaviour using populations of bacteria. We are looking at two main aspects of this concept: self-organisation using Turing pattern formation, and synaptic signal transduction using voltage output glutamate detection (read more...)

Signalling Bacillus
Voltage Modelling

Poster & Presentation Outline


Sponsors


labtech Clontech expressys
invitrogen geneservice cambridge bioscience
zymo research VWR Microzone
Finnzymes Fisher Scientific DNA 2.0


Help


Retrieved from "http://2008.igem.org/Team:Cambridge"