Team:Cambridge/Signalling/Vectors
From 2008.igem.org
(Difference between revisions)
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The integration site of these is pre-determined | The integration site of these is pre-determined | ||
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! BGSC Accession | ! BGSC Accession | ||
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The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there. | The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there. | ||
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! BGSC Accession | ! BGSC Accession | ||
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= Shuttle Vectors = | = Shuttle Vectors = | ||
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! BGSC Accession | ! BGSC Accession | ||
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| [[image:ECE188.jpg|thumb|ECE188 Map]] | | [[image:ECE188.jpg|thumb|ECE188 Map]] | ||
| [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_pHCMC02.htm From Bayreuth] | | [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_pHCMC02.htm From Bayreuth] | ||
- | | | + | | fontcolor="white"| Not yet checked, DNA stocks |
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| ECE189 | | ECE189 | ||
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| [[image:ECE188.jpg|thumb|ECE189 Map]] | | [[image:ECE188.jpg|thumb|ECE189 Map]] | ||
| [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_pHCMC04.htm From Bayreuth] | | [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_pHCMC04.htm From Bayreuth] | ||
- | | | + | | fontcolor="white"| Not yet checked, DNA stocks |
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| ECE190 | | ECE190 |
Revision as of 04:34, 29 October 2008
All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University.
Integration VectorsThese vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or whole-plasmid insertions that only require one crossover and one corresponding region of homology. Ectopic IntegrationThe integration site of these is pre-determined
Insert IntegrationThe user inserts a homologous piece of the Bacillus chromosome and the vector integrates there.
Shuttle Vectors
Plasmid Gels
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