Team:Chiba/Calendar-Home/10 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
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'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
-
::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620　BBa_F2620](2007)).
+
::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620　 BBa_F2620](2007)).
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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</tr>
</tr>
<tr>
<tr>
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<td>dNTP mix(μL)</td>
+
<td>dNTP mix(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Foward Primer(μL)</td>
+
<td>Foward Primer(&mu;L)</td>
<td>0.3</td>
<td>0.3</td>
</tr>
</tr>
<tr>
<tr>
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<td>Reverse Primer(μL)</td>
+
<td>Reverse Primer(&mu;L)</td>
<td>0.3</td>
<td>0.3</td>
</tr>
</tr>
<tr>
<tr>
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<td>taq DNA polymerase(μL)</td>
+
<td>taq DNA polymerase(&mu;L)</td>
<td>0.5</td>
<td>0.5</td>
</tr>
</tr>
<tr>
<tr>
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<td>Thermopol Buffer(μL)</td>
+
<td>Thermopol Buffer(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
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<td>Nuclease free water(μL)</td>
+
<td>Nuclease free water(&mu;L)</td>
<td>20</td>
<td>20</td>
</tr>
</tr>
<tr>
<tr>
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<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>30</td>
<td>30</td>
</tr>
</tr>
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--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]'''
--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]'''
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eluted with 50μL of TE buffer.
+
eluted with 50&mu;L of TE buffer.
===Team:Output===
===Team:Output===

Revision as of 19:34, 29 October 2008

>Home | Notebook

9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->

Colony Count
  1. LuxI
  2. LuxI with LVA
  3. Las
  4. Background(R0010)
  5. Background(C0261)

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620　 BBa_F2620](2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30

??

95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

??

-->Gel Check

--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

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