Team:Chiba/Calendar-Home/13 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
Line 105: Line 105:
===Team:Communication===
===Team:Communication===
:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
::#BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
+
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
-
::#BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
-
::#BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
+
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
{|align="justify"
{|align="justify"
|[[Image:Chiba-0913.JPG]]
|[[Image:Chiba-0913.JPG]]
Line 113: Line 113:
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>1~3</td>
<td>1~3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(μL)</td>
<td>1</td></tr>
<td>1</td></tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(μL)</td>
<td>1</td></tr>
<td>1</td></tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>4</td></tr>
<td>4</td></tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>6</td></tr>
<td>6</td></tr>
</table>
</table>
Line 138: Line 138:
-->'''[[Team:Chiba/protocol/digestion|Digestion]]'''
-->'''[[Team:Chiba/protocol/digestion|Digestion]]'''
-
::#BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
+
::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
-
::#BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
-
::#BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
+
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Line 150: Line 150:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(μL)</td>
<td>5</td><td>5</td><td>1</td><td>1</td>
<td>5</td><td>5</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRⅠ(μL)</td>
<td>0.5</td><td>o.5</td><td>-</td><td>-</td>
<td>0.5</td><td>o.5</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeⅠ(μL)</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 1</td>
+
<td>Buffer 1(μL)</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 3</td>
+
<td>Buffer 3(μL)</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(μL)</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>2</td><td>2</td><td>7.5</td><td>-</td>
<td>2</td><td>2</td><td>7.5</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>10</td><td>10</td><td>10</td><td>1</td>
<td>10</td><td>10</td><td>10</td><td>1</td>
</tr>
</tr>
</table>
</table>
-
 
-
 
-
 
===Team:Output===
===Team:Output===
no work
no work

Revision as of 03:13, 25 October 2008

>Home | Notebook

12 September 2008 <|> 14 September 2008

Contents

Laboratory work

Team:Input

Time Delay Test ①T9002(Ptet) -p15a-Kan(BW)

②Plac-RhlI -Amp(BW)

③Plac-LasI -Amp(BW)

④Ptet-LuxI -Amp(BW)

  • プレ培

①LB-Kan 2ml培養

②、③LB-Amp -0.2%グルコース 2ml培養

④LB-Amp　2ml培養

↓over night

  • 本培

50ml遠心管に①の菌液100μlとLB-Kanを20mlを加える。

50ml遠心管に②、③それぞれの菌液10μlとLB-Amp 0.2%グルコースをそれぞれに6mlを加える。

50ml遠心管に④のLB-Ampを6mlを加える。

OD>2となったらWash


  • ①に関して

①

↓冷却遠心(3700rpm,3min)　　　　　　　　　　

上澄みを捨てる

LB-Kanを20ml加える。　　　　　　　　　　　　


  • ②③④に関して

②、③、④

↓冷却遠心(3700rpm,3min)

上澄みを捨てる。

LBを2ml加える。　　　　　　　　　　　　

↓冷却遠心(3700rpm,3min)

上澄みを捨てる。

LBを2ml加える。

↓冷却遠心(3700rom,3min)

上澄み捨てる。

LB-Ampを6ml加える。


48欠Deep Well

①:2ml ②:2ml,AHL②:2ml③:2ml④2ml-
- -----
①:1ml,②:1ml,IPTG:2μl ①:100μl,②:1ml,IPTG:1.1μl①10μl,②1ml,IPTG:101μl①:1μl,②1ml,IPTG:1.001μl①:1ml,②:100μl,IPTG:1.1μl①:1ml,②:10μl,IPTG:1.01μl
- -----
①:1ml,③:1ml,IPTG:2μl ①:100μl,③:1ml,IPTG:1.1μl①:10μl,③:1ml,IPTG:1.01μl①:1μl,③:1ml,IPTG①:1ml,③:100μl,IPTG:1.1μl①:1ml,③:10μl,IPTG:1.01μl
- -----
①:1ml,④:1ml,IPTG:2μl ①100μl,④:1ml,IPTG:1.1μl①:10μl,④:1ml,IPTG:1.0μl①:1μl,④:1ml,IPTG:1.001μl①:1ml,④:100μl,IPTG:1.0μl①:1ml,④:10μl,IPTG1.01μl
- -----


Team:Communication

Gel Check
  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Chiba-0913.JPG
Sample No. 1~3
Sample DNA(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;


-->Digestion

  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)


Sample No ①②③④
Sample DNA(μL) 5511
EcoRⅠ(μL) 0.5o.5--
SpeⅠ(μL) 0.50.5--
Buffer 1(μL) 11--
Buffer 3(μL) --1-
BSA(μL) 11--
dH2O(μL) 227.5-
TOTAL(μL) 1010101

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/13_September_2008"