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Team:Chiba/Calendar-Home/17 August 2008
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(New page: ===Toggle swich team=== バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026 Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) Insert PCR <br>DNA template ...) |
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| - | + | >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]] | |
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| - | + | [[Team:Chiba/Calendar-Home/16 August 2008|16 August 2008 <]]|[[Team:Chiba/Calendar-Home/18 August 2008|> 18 August 2008]] | |
| - | + | ==Laboratory work== | |
| + | ===Team:Input=== | ||
| + | no work | ||
| - | + | ===Team:Communication=== | |
| - | + | :'''[[Team:Chiba/protocol/transformation|Transformation]]''' | |
| - | + | :competent cells : XL10G | |
| - | + | ::*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] --> No colonies on plates | |
| - | + | ::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] --> No colonies on plates | |
| - | + | ::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] --> No colonies on plates | |
| - | + | ::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] --> No colonies on plates | |
| - | + | ::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] --> No colonies on plates | |
| + | ::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] --> No colonies on plates | ||
| + | ===Team:Output=== | ||
| + | '''[[Team:Chiba/protocol/PCR|PCR]]''' | ||
| + | *[http://partsregistry.org/Part:BBa_E0430 BBa_E0430](1) | ||
| + | *[http://partsregistry.org/Part:BBa_E0420 BBa_E0420](2) | ||
| + | *[http://partsregistry.org/Part:BBa_J06702 BBa_J06702](3) | ||
| + | *[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](4) | ||
| + | *[http://partsregistry.org/Part:BBa_I730003 BBa_I730003](5) | ||
| + | *[http://partsregistry.org/Part:BBa_I730004 BBa_I730004](6) | ||
| + | *[http://partsregistry.org/Part:BBa_I763026 BBa_I763026](7) | ||
| + | *[http://partsregistry.org/Part:BBa_I763002 BBa_I763002](8) | ||
| - | + | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | |
| - | + | <tr> | |
| - | + | <td width="257">Sample No.</td> | |
| + | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA template(μL)</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>FW primer(μL)</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>VR primer(μL)</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dNTPmix(μL)</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>thermo pol buffer(μL)</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA pol(VENT)(μL)</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH<sub>2</sub>O(μL)</td> | ||
| + | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL(μL)</td> | ||
| + | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | ||
| + | </tr> | ||
| + | </table> | ||
Latest revision as of 05:11, 30 October 2008
16 August 2008 <|> 18 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- competent cells : XL10G
- BBa_R0040(2008) --> No colonies on plates
- BBa_C0070(2008) --> No colonies on plates
- BBa_C0076(2008) --> No colonies on plates
- BBa_C0077(2008) --> No colonies on plates
- BBa_C0078(2008) --> No colonies on plates
- BBa_C0079(2008) --> No colonies on plates
Team:Output
- BBa_E0430(1)
- BBa_E0420(2)
- BBa_J06702(3)
- BBa_S01416(4)
- BBa_I730003(5)
- BBa_I730004(6)
- BBa_I763026(7)
- BBa_I763002(8)
| Sample No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| DNA template(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| FW primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| VR primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| dNTPmix(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT)(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O(μL) | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
| TOTAL(μL) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
