Team:Chiba/Calendar-Home/18 August 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 05:13, 30 October 2008

>Home | Notebook

17 August 2008 <|> 19 August 2008

Laboratory work

Team:Input

Transformation

[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

competent cells : XL10Gold(Cm),LB-agar(Kan^R)

Team:communication

PCR

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008)
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008)
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control

DNA Template(μL)	1
dNTP mix(μL)	10
Foward Primer(μL)	5
Reverse Primer(μL)	5
DNA polymerase(μL)	1
Thermopol Buffer(μL)	10
dH₂O(μL)	68
TOTAL(μL)	100

95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

--->Gel Check

Sample DNA	3
Loading Dye(μL)	2
dH₂O(μL)	7
TOTAL(μL)	12

From left;

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008) ->None
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) ->None
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) ->None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) ->None
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008) ->None
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK

Transformation

competent cells : XL10G

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2006) -> No colonies on plates(19/8)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

-->(20/8)Mini prep

Team:Output

no work

@@ Line 1: / Line 1: @@
-'''UV Sensor Team'''
+>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
-バイオブリックパーツおこし
+[[Team:Chiba/Calendar-Home/17 August 2008|17 August 2008 <]]|[[Team:Chiba/Calendar-Home/19 August 2008|> 19 August 2008]]
-<br>BBa_S01416
+==Laboratory work==
-<br>4E-iGEm2006 DNA
+===Team:Input===
-<br>4E-Plate2
-''Transformation''
+'''[[Team:Chiba/protocol/transformation|Transformation]]'''
-<br>z-competent Xl10G(Cm)
+*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
-<br>Plate-LB Kan
+*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)
+competent cells : XL10Gold(Cm),LB-agar(Kan<sup>R</sup>)
+===Team:communication===
+:'''[[Team:Chiba/protocol/PCR|PCR]]'''
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008)
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008)
+::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008)
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008)
+::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008)
+::*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">DNA Template(&mu;L)</td>
+<td>1</td>
+</tr>
+<tr>
+<td>dNTP mix(&mu;L)</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Foward Primer(&mu;L)</td>
+<td>5</td>
+</tr>
+<tr>
+<td>Reverse Primer(&mu;L)</td>
+<td>5</td>
+</tr>
+<tr>
+<td>DNA polymerase(&mu;L)</td>
+<td>1</td>
+</tr>
+<tr>
+<td>Thermopol Buffer(&mu;L)</td>
+<td>10</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>68</td>
+</tr>
+<tr>
+<td>TOTAL(&mu;L)</td>
+<td>100</td>
+</tr>
+</table>
+:95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0818.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye(&mu;L)</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL(&mu;L)</td>
+<td>12</td>
+</tr>
+</table>
+:From left;
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008) ->None
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) ->None
+::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) ->None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) ->None
+::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008) ->None
+::*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK
+|}
+:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+competent cells : XL10G
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2006) -> No colonies on plates(19/8)
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
+:-->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
+===Team:Output===
+no work