Team:Chiba/Calendar-Home/18 August 2008

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17 August 2008 <|> 19 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

Transformation

BBa_S01416(2006)
BBa_S01416(2007)

competent cells : XL10Gold(Cm),LB-agar(Kan^R)

Team:communication

PCR

BBa_C0070(2008)
BBa_C0076(2008)
BBa_C0077(2008)
BBa_C0078(2008)
BBa_C0079(2008)
BBa_F2620(2007)･･･control

DNA Template	1
dNTP mix	10
Foward Primer	5
Reverse Primer	5
DNA polymerase	1
Thermopol Buffer	10
dH₂O	68
TOTAL	100μL

95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

BBa_C0070(2008) ->None
BBa_C0076(2008) ->None
BBa_C0077(2008) ->None
BBa_C0078(2008) ->None
BBa_C0079(2008) ->None
BBa_F2620(2007)･･･control ->OK

Transformation

competent cells : XL10G

BBa_C0070(2007)
BBa_C0070(2006)
BBa_C0076(2007)
BBa_C0076(2006) -> No colonies on plates(19/8)
BBa_C0078(2007)
BBa_C0078(2006)
BBa_C0170(2007)
BBa_C0170(2006)
BBa_C0178(2007)
BBa_C0178(2006)

-->(20/8)Mini prep

Team:Output

no work

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