Team:Chiba/Calendar-Home/20 August 2008
From 2008.igem.org
19 August 2008 <|> 21 August 2008
Contents |
Laboratory work
Team:Input
- BBa_J22136(2007)(1)
- BBa_J22136(2008)(2)
- BBa_J22141(2007)(3)
- BBa_J22141(2008)(4)
- BBa_E0612(2008)(5)
Sample No. | 1 | 2 | 3 | 4 | 5 |
DNA tamplate(μL) | 1 | 1 | 1 | 1 | 1 |
FW primer(μL) | 5 | 5 | 5 | 5 | 5 |
VR primer(μL) | 5 | 5 | 5 | 5 | 5 |
dNTPmix(μL) | 10 | 10 | 10 | 10 | 10 |
thermo pol buffer(μL) | 10 | 10 | 10 | 10 | 10 |
DNA pol(VENT)(μL) | 1 | 1 | 1 | 1 | 1 |
dH2O(μL) | 68 | 68 | 68 | 68 | 68 |
TOTAL(μL) | 100 | 100 | 100 | 100 | 100 |
-->Gel Check
Sample No. | 1 | 2 | 3 | 4 | 5 |
Sample DNA | 1 | 1 | 1 | 1 | 1 |
loading dye(μL) | 1 | 1 | 1 | 1 | 1 |
dH2O(μL) | 4 | 4 | 4 | 4 | 4 |
Total(μL) | 6 | 6 | 6 | 6 | 6 |
-->1,2,3,4,5:none
- BBa_R0051(2007)
- BBa_R0051(2006)
- BBa_J06650(2007)
- BBa_J06650(2006)
- BBa_J22136(2007)
- BBa_J22141(2007)-->only one colony
Team:Communication
- (18/8)--->Mini prep
- --->Gel Check
--->Digestion test
Sample No | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
BBa_C0170(2006) | 3 | - | - | - | 3 | - | - | - |
BBa_C0170(2007) | - | 3 | - | - | - | 3 | - | - |
BBa_C0178(2006) | - | - | 3 | - | - | - | 3 | - |
BBa_C0178(2007) | - | - | - | 3 | - | - | - | 3 |
EcoRI | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
PstI | - | - | - | - | 0.1 | 0.1 | 0.1 | 0.1 |
Buffer EcoRI | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
BSA | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 4.9 | 4.9 | 4.9 | 4.9 | 4.8 | 4.8 | 4.8 | 4.8 |
TOTAL | 10μL | 10μL | 10μL | 10μL | 10μL | 10μL | 10μL | 10μL |
- --->Gel Check
Team:Output
- BBa_J52008(2007)-->colony
- BBa_I712019(2008)-->no colony
- BBa_J33202(2007)-->colony
- BBa_E2030(2007)-->not so good
- YFP(P-GEX)-->colony(Amp)
- BBa_F2620(2007)-->colony
- BBa_T9002(2007)-->colony