Team:Chiba/Calendar-Home/9 September 2008

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8 September 2008 <|> 10 September 2008

Laboratory work

Team:Input

no work

Team:Communication

-->(10/9)

Colony Count
  1. LuxI
  2. LuxI with LVA
  3. Las
  4. Background(R0010)
  5. Background(C0261)

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
DNA Template 1
dNTP mix 5
Foward Primer 0.3
Reverse Primer 0.3
taq DNA polymerase 0.5
Thermopol Buffer 3
Nuclease free water 20
TOTAL 30μL

??

95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

??

-->Gel Check

--->(28/8)Miniprep

elute 50μL

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