Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)
(Week 1)
(Week 1)
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*17 August, 2008  
*17 August, 2008  
:[[Team:Chiba/protocol/transformation|Transformation]]
:[[Team:Chiba/protocol/transformation|Transformation]]
-
::*BBa_[http://partsregistry.org/Part:BBa_R0040| R0040](2008) -> No colonies on plates  
+
::*[http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates  

Revision as of 16:38, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates


  • 18 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


--->(20/8)Mini prep


  • 19 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)・・・control


--->Gel Check
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)・・・control


  • 20 August, 2008
(18/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


--->Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006) -> OK
--->Digestion
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


  • 21 August, 2008
(20/8)--->PCR
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)


--->Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None


Transformation
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)


  • 22 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
(Locate & 2 Punch ~iyama ver.~)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates


  • 23 August, 2008
--->Mini prep
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)


--->Digestion
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)


--->Gel Check
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)

Week 2

  • 24 August, 2008
(22/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
--->Digestion
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
--->Gel Check
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)


  • 25 August, 2008
(24/8)--->Digestion
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_R0010| BBa_R0010](2007)


--->Gel Check
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_R0010| BBa_R0010](2007)


  • 26 August, 2008


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).


  • 30 August, 2008

Week 3

  • 31 August, 2008


  • 1 September, 2008

Week 4

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