Team:Chiba/jk/β

From 2008.igem.org

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Revision as of 12:49, 2 September 2008

β班の実験ノート

Contents

Week 1

  • 17 August, 2008
Transformation
  • BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
  • BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
  • BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
  • BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
  • BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates


  • 18 August, 2008
Transformation
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]


--->(20/8)Mini prep


  • 19 August, 2008
PCR
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control


--->Gel Check
Chiba-0818.JPG
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control ->OK


  • 20 August, 2008
(18/8)--->Mini prep
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]


--->Gel Check
Sample No
Sample DNA 3333
Loading Dye 2222
dH2O 7777
TOTAL 12121212
Chiba-0820.JPG

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

Chiba-0820-2.JPG
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
Chiba-0820-3.JPG
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
Chiba-0820-4.JPG
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK


--->Digestion
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]


  • 21 August, 2008
(20/8)--->PCR
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]


--->Gel Check
Chiba-0821.JPG

From the left;

  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK


Transformation
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]


  • 22 August, 2008
Transformation
  • BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
  • BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
(Locate & 2 Punch ~iyama ver.~)
  • BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
  • BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates



  • 23 August, 2008
--->Mini prep
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]


--->Digestion
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]


--->Gel Check</table>
Chiba-0820.JPG

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

Chiba-0823-1.JPG
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
Chiba-0823-2.JPG
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • M
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
Chiba-0823-3.JPG
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion


Week 2

  • 24 August, 2008
(22/8)--->Mini prep
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
--->Digestion
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
--->Gel Check
Sample No
Sample DNA 11111111
Loading Dye 11111111
dH2O 44444444
TOTAL 66666666
Chiba-0824-1.JPG

From the left;

①BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
②BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
④BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

(↓single digestuion)

⑤BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
⑦BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
Sample No
Sample DNA 9999
Loading Dye 2222
dH2O 1111
TOTAL 12121212
Chiba-0824-2.JPG

From the left; (single digestuion)

⑤BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
⑦BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • 25 August, 2008
(24/8)--->Digestion
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
  • BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)


--->Gel Check
Sample No
Sample DNA 9999
Loading Dye 2222
dH2O 1111
TOTAL 12121212
Chiba-0825-1.JPG

From left;

Chiba-0825-2.JPG

From left;

Chiba-0825-3.JPG

From left;

Chiba-0825-4.JPG

From left;

Chiba-0825-5.JPG

From left;

  • 26 August, 2008
--->Gel Check
Sample No
Sample DNA 9999
Loading Dye 2222
dH2O 1111
TOTAL 12121212
Chiba-0826.JPG

From left;


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).


  • 30 August, 2008

Week 3

  • 31 August, 2008
  • 1 September, 2008
  • 2 September, 2008
  • 3 September, 2008
  • 4 September, 2008
  • 5 September, 2008
  • 6 September, 2008


Week 4

  • 7 September, 2008
  • 8 September, 2008
  • 9 September, 2008
  • 10 September, 2008


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