Team:Chiba/jk/γ/week 4

From 2008.igem.org

(Difference between revisions)

Latest revision as of 06:52, 24 October 2008

実験ログ
 出力班

Week 4

7,September,2008

Cloning

Digestion

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)	50	54	54	45	14	54
SpeⅠ	1	0	0	0	1	0
PstⅠ	0.5	0.5	0.5	0.5	0.5	0.5
XbaⅠ	0	0.5	0.5	0.5	0	0.5
BSA(x10)	0.6	7	7	6	2	7
Buffer2	6	-	-	-	2	-
Buffer3	-	7	7	6	-	7
dH₂O	1.9	1	1	2	0.5	1
TOTAL	60	70	70	60	20	70

-->37℃ 3hour

-->Gel Extraction

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)	6	10	10	10	4	10
Loading Dye	2	2	2	2	1	2
dH₂O	4	-	-	-	1	2
TOTAL	12	12	12	12	6	12
well	10	7	7	6	5	7

-->DNA purification (①-④,⑥:7μl ⑤:6μl)

8,Septmeber,2008

SAP

[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤

Sample No.	①	⑤
DNA(digested sample)	7	6
SAP/td>	2	2
SAP Buffer	3	3
TOTAL	30	30
TOTAL	30	30

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check

Sample No.	each
DNA(digested sample)	1
Loading Dye	1
dH₂O	4
TOTAL	6

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl

Sample No.	①&②	①&③	①&④	①	⑤&⑥	⑤
DNA(vector)	3	3	3	3	5	2.1
DNA(insert)	3	3	3	-	3.1	-
Ligase	1	1	1	1	1	1
Ligase Buffer(x5)	2	2	2	2	2	2
dH₂O	1	1	1	4	8.9	4
TOTAL	10	10	10	10	20	10

9,September,2008

10,September,2008

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

11,September,2008

12,September,2008

13,September,2008

>Last Week
>Next Week

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 1: / Line 1: @@
-[[team:Chiba/jk|実験ログ]]
+[[Team:Chiba/jk|実験ログ]]
+<br>[[Team:Chiba/jk/γ|出力班]]
 == Week 4 ==
@@ Line 6: / Line 6: @@
 == 7,September,2008 ==
-<br>Cloning
+*Cloning
-<br>Digestion
+[[Team:Chiba/protocol/digestion|Digestion]]
-<br>vector:BBa_R0040(ptet)① 75ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
-<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
-<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
-<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
-<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
-<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample)</td>
 <td>50</td><td>54</td><td>54</td><td>45</td><td>14</td><td>54</td>
 </tr>
@@ Line 48: / Line 49: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
 </tr>
@@ Line 56: / Line 57: @@
 </tr>
 </table>
-<br>----->37℃ 3hour
-<br>----->Gel Extraction
+-->37℃ 3hour
+-->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample)</td>
 <td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
 </tr>
@@ Line 72: / Line 76: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
 </tr>
@@ Line 83: / Line 87: @@
 <td>10</td><td>7</td><td>7</td><td>6</td><td>5</td><td>7</td>
 </table>
-<br>->DNA purification->①-④,⑥->7μl ⑤->6μl
+-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (①-④,⑥:7μl ⑤:6μl)
 == 8,Septmeber,2008 ==
-<br>SAP
+[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-<br>BBa_R0040(ptet)①
+*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
-<br>BBa_R0010(pLac)⑤
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>⑤</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample)</td>
 <td>7</td><td>6</td>
 </tr>
@@ Line 117: / Line 123: @@
 </tr>
 </table>
-<br>->37℃ 1hour
-<br>->65℃ 30min
+-->37℃ 1hour
-<br>->DNA purification
-<br>->21μl  6μl
+-->65℃ 30min
-<br>->Gel cheack
+-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]]
+-->21μl  6μl
+-->[[Team:Chiba/protocol/gelcheck|Gel check]]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>each</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample)</td>
 <td>1</td>
 </tr>
@@ Line 136: / Line 148: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td>
 </tr>
@@ Line 144: / Line 156: @@
 </tr>
 </table>
-<br>vector:BBa_R0040(ptet)① 75ng/μl
-<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
-<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
-<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
-<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
-<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
+*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
 </tr>
@@ Line 172: / Line 186: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
@@ Line 187: / Line 202: @@
 == 10,September,2008 ==
-<br>TIME Responce(liquid)
+[[Team:Chiba/jk/γ/Trl|TIME Responce(liquid)]]
-<br>TIME Responce(solid)
-<br>Colony PCR
-<br>->Gel cheack
-<br>MINI Prep
+[[Team:Chiba/jk/γ/trs|TIME Responce(solid)]]
-== 13,September,2008 ==
+[[Team:Chiba/protocol/PCR|Colony PCR]]
+-->[[Team:Chiba/protocol/gelcheck|Gel check]]
+-->[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]]
+== 11,September,2008 ==
+==12,September,2008==
+== 13,September,2008 ==
@@ Line 201: / Line 225: @@
+<br>[[Team:Chiba/jk/γ/week 3|>Last Week]]
+<br>[[Team:Chiba/jk/γ/week 5|>Next Week]]

Team:Chiba/jk/γ/week 4

From 2008.igem.org

Latest revision as of 06:52, 24 October 2008

Contents

Week 4

7,September,2008

8,Septmeber,2008

9,September,2008

10,September,2008

11,September,2008

12,September,2008

13,September,2008