Team:Chiba/protocol/Time Delay Test

From 2008.igem.org

(Difference between revisions)
 
Line 8: Line 8:
#Washed  sender and receiver.
#Washed  sender and receiver.
#Mixed them. (Sender:Receiver=1000μL:1000μL)
#Mixed them. (Sender:Receiver=1000μL:1000μL)
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#Incubated at 30°C.
+
#Incubated at 25°C, 30°C or 37°C.
#Measured intensity of green fluorescence at regular time intervals.(Fluoroskan Ascent<sup>R</sup> FL&Fluoroskan Ascent<sup>R</sup> Thermo ELECTRON CORPORATION)
#Measured intensity of green fluorescence at regular time intervals.(Fluoroskan Ascent<sup>R</sup> FL&Fluoroskan Ascent<sup>R</sup> Thermo ELECTRON CORPORATION)

Latest revision as of 06:38, 26 October 2008

>Protcol

Time Delay Test

  1. Transformed sender and receiver into E coli strains.
  2. Inoculated them independently in liquid media. Incubated at 37℃ 12h.
  3. Inoculated again in Fresh liquid media upto about OD600=2 at 37℃
  4. Washed sender and receiver.
  5. Mixed them. (Sender:Receiver=1000μL:1000μL)
  6. Incubated at 25°C, 30°C or 37°C.
  7. Measured intensity of green fluorescence at regular time intervals.(Fluoroskan AscentR FL&Fluoroskan AscentR Thermo ELECTRON CORPORATION)