Team:Edinburgh/GBS1

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GBS1

For better alignment see .txt file

AMINO ACID SEQUENCE

MATvTASSNFvSRTSLFNNHGASSCSDVAQITLKGQSlTHCGLRSFNMvD
NLQRRSQAKPVSAKSSKRSSKVKTAGKIvCEKGMSvIFIGAEVGPWSKTG
GLGDVLGGLPPALAARGHRvMTICPRYDQYKDAWDTCVvVQIKVGDKVEN
VRFFHCYKRGVDRVFVDHPIFLAKVvGKTGSKIYGPITGVDYNDNQLRFS
llCQAALEAPQVlNlNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQ
SRGVYMNAKvVFCIHNIAYQGRFAFDDYSLLNlPISFKSSFDFMDGYEKP
VKGRKINWMKAAIlEAHRVLTVSPYYAQELISGVDRGvElHKYLRMKTVS
GIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPlIKEALQAAVGLPvDRD
VPVIGFIGRlEEQKGSDILvEAISKFMGLNVQMVILGTGKKKMEAQILEL
EEKFPGKAVGvAKFNvPlAHMITAGADFIIVPSRFEPCGLIQlHAMRYGT
VPIvASTGGLvDTvKDGYTGFHIGRFNVKCEVvDPDDvIATAKAvTRAVA
VYGTSAMQEMVKNCMDQDFSWKGPARlWEKVLlSLNvAGSEAGTEGEEIA
PlAKENVATP*

DNA SEQUENCE

atggcaactgtgactgcttcttctaactttgtgtcaagaacttcactttt 50
taccgttgacactgacgaagaagattgaaacacagttcttgaagtgaaaa

caacaatcatggtgcttcttcatgctctgatgtcgctcagattaccttaa 100
gttgttagtaccacgaagaagtacgagactacagcgagtctaatggaatt

aaggccaatccttgactcattgtgggttaaggtcattcaacatggtggat 150
ttccggttaggaactgagtaacacccaattccagtaagttgtaccaccta

aaccttcagaggagatctcaagctaaacctgtttctgctaaatcctcaaa 200
ttggaagtctcctctagagttcgatttggacaaagacgatttaggagttt

gagatcttctaaagttaagactgctggtaagattgtgtgtgagaaaggaa 250
ctctagaagatttcaattctgacgaccattctaacacacactctttcctt

tgtctgtgatttttattggagctgaagttggtccatggagtaaaactggt 300
acagacactaaaaataacctcgacttcaaccaggtacctcattttgacca

ggtcttggtgatgttctcggtggtctacctccagctcttgctgctagagg 350
ccagaaccactacaagagccaccagatggaggtcgagaacgacgatctcc

ccaccgtgtgatgacaatttgtcctcggtatgaccaatataaagatgctt 400
ggtggcacactactgttaaacaggagccatactggttatatttctacgaa

gggacacttgtgttgtggttcagatcaaagttggggataaagttgagaat 450
ccctgtgaacacaacaccaagtctagtttcaacccctatttcaactctta

gttcgtttcttccattgctacaaacgaggagttgatcgtgtctttgttga 500
caagcaaagaaggtaacgatgtttgctcctcaactagcacagaaacaact

ccatccaatctttcttgctaaggttgtgggcaaaacaggatccaaaatct 550
ggtaggttagaaagaacgattccaacacccgttttgtcctaggttttaga

atggtcctataactggagtagactacaatgacaaccaactccggttcagt 600
taccaggatattgacctcatctgatgttactgttggttgaggccaagtca

ttgttgtgtcaggctgctcttgaggcaccacaggttctgaacctgaacag 650
aacaacacagtccgacgagaactccgtggtgtccaagacttggacttgtc

cagcaagtacttctctggaccatatggtgaagatgtagtctttgttgcca 700
gtcgttcatgaagagacctggtataccacttctacatcagaaacaacggt

atgactggcacactgctctacttccatgttacctcaaatctatgtatcaa 750
tactgaccgtgtgacgagatgaaggtacaatggagtttagatacatagtt

tcccgcggagtctacatgaatgcaaaggtggtcttctgcattcacaacat 800
agggcgcctcagatgtacttacgtttccaccagaagacgtaagtgttgta

agcctaccagggaagatttgcctttgatgactattcccttctcaacttgc 850
tcggatggtcccttctaaacggaaactactgataagggaagagttgaacg

ccatcagctttaaaagttctttcgacttcatggacgggtatgaaaagcca 900
ggtagtcgaaattttcaagaaagctgaagtacctgcccatacttttcggt

gtaaaaggacggaaaattaactggatgaaggctgcaattctggaagctca 950
cattttcctgccttttaattgacctacttccgacgttaagaccttcgagt

ccgtgtcttaacagttagtccatactacgctcaagaactcatctctggag 1000
ggcacagaattgtcaatcaggtatgatgcgagttcttgagtagagacctc
v ttgatagaggcgtggaattgcataaatatcttcgaatgaaaacagtttcc 1050
aactatctccgcaccttaacgtatttatagaagcttacttttgtcaaagg

ggaattattaatggaatggatgttcaagaatggaacccgtctactgacaa 1100
ccttaataattaccttacctacaagttcttaccttgggcagatgactgtt

gtacatcgatatcaaatacgatattaccactgttacagatgctaaaccat 1150
catgtagctatagtttatgctataatggtgacaatgtctacgatttggta

tgatcaaagaagcacttcaggctgctgttggacttcccgtggacagggat 1200
actagtttcttcgtgaagtccgacgacaacctgaagggcacctgtcccta

gtcccggttatcggtttcatagggagattggaggagcagaagggttctga 1250
cagggccaatagccaaagtatccctctaacctcctcgtcttcccaagact

tattctagtggaagctatttccaagttcatggggctcaatgttcagatgg 1300
ataagatcaccttcgataaaggttcaagtaccccgagttacaagtctacc

ttatccttgggactggaaagaagaagatggaggctcagattcttgaacta 1350
aataggaaccctgacctttcttcttctacctccgagtctaagaacttgat

gaagagaagttcccagggaaggcggttggagtggcgaaattcaacgtgcc 1400
cttctcttcaagggtcccttccgccaacctcaccgctttaagttgcacgg

attggctcatatgatcactgctggagctgacttcatcattgtcccaagca 1450
taaccgagtatactagtgacgacctcgactgaagtagtaacagggttcgt

ggtttgagccgtgtggtctcattcagctgcacgcaatgagatatggaacc 1500
ccaaactcggcacaccagagtaagtcgacgtgcgttactctataccttgg

gtccctattgtggcatctactggtggacttgtggacactgtgaaagatgg 1550
cagggataacaccgtagatgaccacctgaacacctgtgacactttctacc

ctacacaggtttccacattggaagattcaacgtcaagtgtgaagttgtgg 1600
gatgtgtccaaaggtgtaaccttctaagttgcagttcacacttcaacacc

atccagatgatgtgatagcaacagcaaaggctgtgacaagagccgttgca 1650
taggtctactacactatcgttgtcgtttccgacactgttctcggcaacgt

gtatatggaacatccgcaatgcaagaaatggtcaagaactgcatggacca 1700
catataccttgtaggcgttacgttctttaccagttcttgacgtacctggt

agacttctcctggaagggacctgcgaggttgtgggagaaggtactattgt 1750
tctgaagaggaccttccctggacgctccaacaccctcttccatgataaca

cccttaatgtggcgggaagtgaagccggaaccgagggtgaagagatagct 1800
gggaattacaccgcccttcacttcggccttggctcccacttctctatcga

cctctggccaaggagaacgtagcgacgccgtga 1833
ggagaccggttcctcttgcatcgctgcggcact

No forbidden restriction sites!

BIOBRICKTM PRIMERS

Forward: CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT (54ºC)
Reverse: CGT t actagt a TTA TTA CGG CGT CGC TAC G (52ºC)