Team:Edinburgh/Notebook

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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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For more information, please see [https://www.wiki.ed.ac.uk/display/iGEM2008/HOME our internal wiki].
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== Calendar ==
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= Calender =
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Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox.
<table>
<table>
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
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<h3>Calendar</h3>
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=06}}
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=07}}
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=08}}
 
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</td></tr>
 
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</table>
</table>
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== Summary of samples: ==
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{| align="center"
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==== PCR products ====
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|-valign="top"
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|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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'''P1:''' ''dxs'' from ''Eschaerichia coli'' JM109 (or K12?)<br />
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|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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'''P2:''' ''appY'' from ''E. coli'' JM109 (or K12?)<br />
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|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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'''P3:''' ''glgC'' from ''E. coli'' JM109 (or K12?)<br />
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|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=09}}
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'''P4:''' fusion PCR for BABEL1+''glgC''<br />
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|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=10}}
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'''P5:''' fusion PCR for BABEL2+''glgC''<br />
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|}
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'''P6:''' mutagenic PCR to remove EcoRI site 1 from ''glgC''<br />
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'''P7:''' mutagenic PCR to remove EcoRI site 2 from ''glgC''<br />
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'''P8:''' ''crtB'' (from ''Pantoea ananatis'' cells)<br />
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'''P9:''' ''crtI'' (from Douglas's maxiprep of the mutated ''crtIB'' plasmid)<br />
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'''P10:''' ''crtY'' (from ''P. ananatis'' cells). Failed.<br />
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'''P11:''' fusion PCR for rbs+''dxs''. Failed.<br />
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'''P12:''' repeat fusion PCR for rbs+''dxs'', using L11 and primers rbs2fclon1+pSB1A2insr1.<br />
+
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'''P13 and P14:''' MABEL mutation of site 1 on ''glgC'' mutant clones M19 and M22.<br />
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'''P15:''' fusion PCR for rbs+''appY''<br />
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'''P16, P17, P18 and P19:''' first attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
+
-
'''P20, P21, P22 and P23:''' second attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
+
-
'''P24, P25:''' repeat PCR for ''cenA'' and ''cex'' using Pfu. Failed.<br />
+
-
'''P26:''' Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene ''rrnB'' from ''Cellulomonas fimi''.<br />
+
-
'''P27:''' ''crtY'' from ''P. ananatis'' cells. - Failed<br />
+
-
'''P28, P29:''' ''crtB'' and ''crtI'' from L18 and L19.<br />
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-
'''P30:''' Repeat positive control ''rrnB'' from ''C. fimi'', annealing 1 minute.<br />
+
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'''P31:''' pZntA promoter<br />
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'''P32-P35:''' repeat PCR for ''cenA'', ''cenB'', ''cenC'' and ''cex'', annealing 1 minute. Failed.<br />
+
-
'''P36:''' M43 (''glgC''-mut1,2) with primers glgCf2/glgCr2 (25.07.08: AM)<br />
+
-
'''P37:''' ''crtY'' from ''P. ananatis''. (25.07.08: AM) - Failed<br />
+
-
'''P38~P41:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from heat-killed cell suspension.<br />
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-
'''P42~P45:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from 'impure' DNA solution.<br />
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-
'''P46~P47:''' ''cenA'' and ''cex'' from 'impure' DNA solution, annealing 65C. Failed.<br />
+
-
'''P48:''' ''crtY'' (new primer mixture) (previously P38a) (30.07.08: CF) - Failed<br />
+
-
'''P49:''' Recreation of P12 (rbs+''dxs'') (previously P39a) (30.07.08: CF)<br />
+
-
'''P50:''' third mutagenesis of ''glgc''-mut1,2 (04.08.08: YAN)<br />
+
-
'''P51:''' pCstA from ''E. coli'' cells (06.08.08: CF) - Failed<br />
+
-
'''P52:''' ''cenA'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
+
-
'''P53:''' ''cex'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
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-
'''P54:''' pCstA from ''E. coli'' cells - Purified (07.08.08: CF)<br />
+
-
 
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-
==== Ligations ====
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'''L1:''' Edinbrick1+''dxs''<br />
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'''L2:''' Edinbrick1+''appY''<br />
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-
'''L3:''' Babel1+''glgC''<br />
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'''L4:''' Babel2+''glgC''<br />
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-
'''L5:''' Edinbrick1+''appY'' repeat (initially labelled L4 in error)<br />
+
-
'''L6:''' Self ligation of mutagenic PCR P6<br />
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'''L7:''' Self ligation of mutagenic PCR P7<br />
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-
'''L8:''' Ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
+
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'''L9:''' Edinbrick1+''crtB''<br />
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'''L10:''' Edinbrick1+''crtI'' (mutant lacking PstI sites, I hope)<br />
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'''L11:''' repeat ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
+
-
'''L12:''' rbs ligated with ''appY''.<br />
+
-
'''L13 and L14:''' self ligations of ''glgC'' mutation reactions P13 and P14.<br />
+
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'''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). - Failed<br />
+
-
'''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17/07/08) - Failed<br />
+
-
'''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br />
+
-
'''L18:''' Ligation of ''crtI'' (M42) to rbs (22/07/08)<br />
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-
'''L19:''' pSB1A2+rbs+''dxs'' ligation product (From P12) (25/07/08: Yan/OG)<br />
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-
'''L20:''' pSB1A2+rbs+''appY'' ligation product (From P15) (25/07/08: Yan/OG)<br />
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-
'''L21:''' pSB1A2+rbs+''crtB'' ligation product (From P28) (25/07/08: Yan/OG)<br />
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-
'''L22:''' pSB1A2+rbs+''crtI'' ligation product (From P29) (25/07/08: Yan/OG)<br />
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-
'''L23:''' PzntA+pSB1A2 ligation product (From P31) (25/07/08: Yan/OG)<br />
+
-
'''L24:''' Ligation of ''crtE'' to pSB1A2 (25/07/08: CF)<br />
+
-
'''L25:''' Ligation of ''glgC'' double mutant (P36) to Edinbrick 1 (28.07.2008: AM)<br />
+
-
'''L26:''' Ligation of Edi+P15 XP - rbs+''appY'' (30.07.2008: HX)<br />
+
-
'''L27:''' Ligation of Edi+P28 XP - rbs+''crtB'' (30.07.2008: HX)<br />
+
-
'''L28:''' Putative ''cenA'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
-
'''L29:''' Putative ''cex'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
-
'''L30:''' Self-ligation of P15 (3rd mutagenesis of ''glgC''-mut1,2) (05.08.08: HX)<br />
+
-
'''L31:''' Ligation of P36 (''glgC''-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)<br />
+
-
'''L32:''' Ligation of rbs+''crtB'' (M67) to rbs+''crtI'' (M50) to create ''crtB''/''crtI'' (07.08.08: AM, Yan)<br />
+
-
'''L33:''' Ligation of PcstA (P54) to Edinbrick1 (08.08.08: OG)<br />
+
-
 
+
-
==== Minipreps ====
+
-
'''M1 to M6:''' pSB1A2+''dxs'' transformants. *M2, M3, M4 and M6* all looked correct on a gel. *M2* was sequenced to confirm identity and was maxiprepped as *X2*.<br />
+
== Summary of samples ==
-
'''M7:''' Babel1+''glgC'' transformant. Did not look right on gel.<br />
+
-
'''M8 to M12:''' Babel2+''glgC''_ transformants. *M10 and M11* both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation.<br />
+
-
'''M13 to M18:''' pSB1A2+''appY'' transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size.<br />
+
-
'''M19 to M24:''' Babel2+''glgC'' clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site.<br />
+
-
'''M25 to M30:''' possible rbs+''dxs'' transformants.<br />
+
-
'''M31 to M36:''' ''crtB'' clones.<br />
+
-
'''M37 to M42:''' ''crtI'' clones.<br />
+
-
'''M43 to M47:''' possible ''glgC'' double mutants (both EcoRI sites gone)<br />
+
-
'''M48:''' a possible rbs+''dxs'' clone.<br />
+
-
'''M49 to M54:''' pSB1A2-rbs+''crtI''<br />
+
-
'''M55 to M60:''' PZntA promoter<br />
+
-
'''M61 to M66:''' BABEL2+rbs+''crtE''<br />
+
-
'''M67:''' rbs+''crtB''<br />
+
-
'''M68 to M71:''' pSB1A2+''glgC''-mut1,2 (31.07.08: AM, YAN)<br />
+
-
'''M72 to M75:''' pSB1A2+rbs+''dxs'' (31.07.08: AM, YAN)<br />
+
-
'''M76 to M79:''' BABEL2+''crtE'' (31.07.08: AM, YAN)<br />
+
-
'''M80 to M83:''' pSB1A2+rbs+''appY'' (04/08/08: YAN and OG)<br />
+
-
'''M84 to M85:''' pSB1A2+rbs+''crtB'' (04/08/08: YAN and OG)<br />
+
-
'''M86 to M90:''' pSB1A2+''cenA'' (04/08/08: YAN and OG)<br />
+
-
'''M91 to M96:''' pSB1A2+''cex''(04/08/08: YAN and OG)<br />
+
-
'''M97 to M104:''' pSB1A2+''cenA'' (05.08.08: YAN)<br />
+
-
'''M105 to M108:''' pSB1A2+''cex'' (05.08.08: YAN)<br />
+
-
==== Maxipreps ====
+
Up-to-date to end 28 October.
-
'''X1:''' BioBrick J33201<br />
+
[[Edinburgh/Notebook/PCR products|PCR products]]<br />
-
'''X2:''' pSB1A2+''dxs'' clone (as M2; BBa_K118000)<br />
+
[[Edinburgh/Notebook/Ligation products|Ligation products]]<br />
-
'''X3:''' pSB1A2+''appY'' (from Plate 21, streak 3) (18 July 2008)<br />
+
[[Edinburgh/Notebook/Minipreps|Minipreps]]<br />
-
'''X4:''' pSB1A2+''crtB'' (as M36; BBa_K118002) (25.07.08: A
+
[[Edinburgh/Notebook/Maxipreps|Maxipreps]]<br />
 +
[[Edinburgh/Notebook/Plates|Plates]]<br />

Latest revision as of 00:26, 30 October 2008

Calendar

Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox.

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16] [http://2008.igem.org/Edinburgh/17_June_2008 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18] [http://2008.igem.org/Edinburgh/19_June_2008 19] [http://2008.igem.org/Edinburgh/20_June_2008 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23] [http://2008.igem.org/Edinburgh/24_June_2008 24] [http://2008.igem.org/Edinburgh/25_June_2008 25] [http://2008.igem.org/Edinburgh/26_June_2008 26] [http://2008.igem.org/Edinburgh/27_June_2008 27] [http://2008.igem.org/Edinburgh/28_June_2008 28] [http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Edinburgh/1_July_2008 1] [http://2008.igem.org/Edinburgh/2_July_2008 2] [http://2008.igem.org/Edinburgh/3_July_2008 3] [http://2008.igem.org/Edinburgh/4_July_2008 4] [http://2008.igem.org/Edinburgh/5_July_2008 5] [http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7] [http://2008.igem.org/Edinburgh/8_July_2008 8] [http://2008.igem.org/Edinburgh/9_July_2008 9] [http://2008.igem.org/Edinburgh/10_July_2008 10] [http://2008.igem.org/Edinburgh/11_July_2008 11] [http://2008.igem.org/Edinburgh/12_July_2008 12] [http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14] [http://2008.igem.org/Edinburgh/15_July_2008 15] [http://2008.igem.org/Edinburgh/16_July_2008 16] [http://2008.igem.org/Edinburgh/17_July_2008 17] [http://2008.igem.org/Edinburgh/18_July_2008 18] [http://2008.igem.org/Edinburgh/19_July_2008 19] [http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21] [http://2008.igem.org/Edinburgh/22_July_2008 22] [http://2008.igem.org/Edinburgh/23_July_2008 23] [http://2008.igem.org/Edinburgh/24_July_2008 24] [http://2008.igem.org/Edinburgh/25_July_2008 25] [http://2008.igem.org/Edinburgh/26_July_2008 26] [http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28] [http://2008.igem.org/Edinburgh/29_July_2008 29] [http://2008.igem.org/Edinburgh/30_July_2008 30] [http://2008.igem.org/Edinburgh/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Edinburgh/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2] [http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4] [http://2008.igem.org/Edinburgh/5_August_2008 5] [http://2008.igem.org/Edinburgh/6_August_2008 6] [http://2008.igem.org/Edinburgh/7_August_2008 7] [http://2008.igem.org/Edinburgh/8_August_2008 8] [http://2008.igem.org/Edinburgh/9_August_2008 9] [http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11] [http://2008.igem.org/Edinburgh/12_August_2008 12] [http://2008.igem.org/Edinburgh/13_August_2008 13] [http://2008.igem.org/Edinburgh/14_August_2008 14] [http://2008.igem.org/Edinburgh/15_August_2008 15] [http://2008.igem.org/Edinburgh/16_August_2008 16] [http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18] [http://2008.igem.org/Edinburgh/19_August_2008 19] [http://2008.igem.org/Edinburgh/20_August_2008 20] [http://2008.igem.org/Edinburgh/21_August_2008 21] [http://2008.igem.org/Edinburgh/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25] [http://2008.igem.org/Edinburgh/26_August_2008 26] [http://2008.igem.org/Edinburgh/27_August_2008 27] [http://2008.igem.org/Edinburgh/28_August_2008 28] [http://2008.igem.org/Edinburgh/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]
September
MTWTFSS
[http://2008.igem.org/Edinburgh/1_September_2008 1] [http://2008.igem.org/Edinburgh/2_September_2008 2] [http://2008.igem.org/Edinburgh/3_September_2008 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_September_2008&action=edit 4] [http://2008.igem.org/Edinburgh/5_September_2008 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_September_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/8_September_2008&action=edit 8] [http://2008.igem.org/Edinburgh/9_September_2008 9] [http://2008.igem.org/Edinburgh/10_September_2008 10] [http://2008.igem.org/Edinburgh/11_September_2008 11] [http://2008.igem.org/Edinburgh/12_September_2008 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_September_2008&action=edit 14]
[http://2008.igem.org/Edinburgh/15_September_2008 15] [http://2008.igem.org/Edinburgh/16_September_2008 16] [http://2008.igem.org/Edinburgh/17_September_2008 17] [http://2008.igem.org/Edinburgh/18_September_2008 18] [http://2008.igem.org/Edinburgh/19_September_2008 19] [http://2008.igem.org/wiki/index.php?title=Edinburgh/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_September_2008&action=edit 21]
[http://2008.igem.org/Edinburgh/22_September_2008 22] [http://2008.igem.org/Edinburgh/23_September_2008 23] [http://2008.igem.org/Edinburgh/24_September_2008 24] [http://2008.igem.org/Edinburgh/25_September_2008 25] [http://2008.igem.org/Edinburgh/26_September_2008 26] [http://2008.igem.org/Edinburgh/27_September_2008 27] [http://2008.igem.org/Edinburgh/28_September_2008 28]
[http://2008.igem.org/Edinburgh/29_September_2008 29] [http://2008.igem.org/Edinburgh/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Edinburgh/1_October_2008 1] [http://2008.igem.org/Edinburgh/2_October_2008 2] [http://2008.igem.org/Edinburgh/3_October_2008 3] [http://2008.igem.org/Edinburgh/4_October_2008 4] [http://2008.igem.org/Edinburgh/5_October_2008 5]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/6_October_2008&action=edit 6] [http://2008.igem.org/Edinburgh/7_October_2008 7] [http://2008.igem.org/Edinburgh/8_October_2008 8] [http://2008.igem.org/Edinburgh/9_October_2008 9] [http://2008.igem.org/Edinburgh/10_October_2008 10] [http://2008.igem.org/Edinburgh/11_October_2008 11] [http://2008.igem.org/Edinburgh/12_October_2008 12]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/13_October_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Edinburgh/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Edinburgh/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Edinburgh/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_October_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Edinburgh/25_October_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Edinburgh/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Edinburgh/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Edinburgh/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_October_2008&action=edit 31]

Summary of samples

Up-to-date to end 28 October.

PCR products
Ligation products
Minipreps
Maxipreps
Plates

Retrieved from "http://2008.igem.org/Team:Edinburgh/Notebook"