"
Team:Hawaii/Antibiotic test for aadA construct
From 2008.igem.org
Revision as of 02:04, 27 October 2008 by MargaretRuzicka (Talk | contribs)
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
Antibiotic test for aadA construct
- The gene for Sp and Sm resistance was constructed in BioBrick format. It was amplified from pRL1383a with primers adding BioBrick ends, and the lac promoter and rbs were ligated to it.
- This construct was tested for antibiotic resistance.
Methods
- Sequencing confirmation
- Streak on LB+Agar plates with Sm50Sp10
- constructs
- (+) control pSMC121 (contains aadA gene)
- (-) control pSB1A3
Results
- Sequencing confirmation
- Streak
The plate streaked with multiple aadA constructs + controls.
- All four of the constructs grew and the positive control grew while the negative control did not grow.
Discussion
- What was learned and how to do future experiments differently.
