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Team:Hawaii/Meeting/2008-06-30
From 2008.igem.org
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Normanwang (Talk | contribs) (→Agenda: misc additions) |
Normanwang (Talk | contribs) (updated with comment from GK) |
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# PCR Week! (our 38 primers arrived last Friday.) What else do we need? | # PCR Week! (our 38 primers arrived last Friday.) What else do we need? | ||
## Template DNA: [http://partsregistry.org/wiki/index.php/Part:pSB1A3 pSB1A3]? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site) | ## Template DNA: [http://partsregistry.org/wiki/index.php/Part:pSB1A3 pSB1A3]? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site) | ||
| + | ##*GK: Actually, it's not pSB1A3 (RE site problems). The primers will work with any Biobrick plasmid, like pSB1A2 (no RE problems) or pSB2K3. Neither have the ccdB gene and are available on plates 1003/4A and 1014/1F, respectively. | ||
## Template DNA: Anabaena cells? to PCR out Nir promoter | ## Template DNA: Anabaena cells? to PCR out Nir promoter | ||
# BioBrick Extracted Roundup | # BioBrick Extracted Roundup | ||
Revision as of 11:05, 30 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
Agenda
- PCR Week! (our 38 primers arrived last Friday.) What else do we need?
- Template DNA: pSB1A3? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site)
- GK: Actually, it's not pSB1A3 (RE site problems). The primers will work with any Biobrick plasmid, like pSB1A2 (no RE problems) or pSB2K3. Neither have the ccdB gene and are available on plates 1003/4A and 1014/1F, respectively.
- Template DNA: Anabaena cells? to PCR out Nir promoter
- Template DNA: pSB1A3? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site)
- BioBrick Extracted Roundup
- summarize what we extracted and update their status (here, and relocate it to the public Wiki)
- cryostocked
- plasmid prepped
- sequence verified
- summarize what we extracted and update their status (here, and relocate it to the public Wiki)
- Revisit Project Description (due Tuesday 7/1)
- Inventory Walk-through (4C Fridge organization, -20C Freezer shelf organization, -80C Freezer shelf organization)
- 2nd Round of Primer Ordering (review and finalize list)
Minutes
Present: <Person A>, <Person B>
Action Items
- <Person A>: Clean up seed PCC6803 seed stock area (many of them are turning yellow and needs to be thrown away)
Coming Up
- 7/1 Project Description Due
