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Team:Hawaii/Meeting/2008-06- 5
From 2008.igem.org
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== Minutes == | == Minutes == | ||
| - | Present: GK, KS, MR, SC, GP, | + | Present: GK, KS, MR, SC, GP, KLS, NW, LG <br> |
| - | Presentations: | + | Presentations:<br> |
Grace: lux operon night light | Grace: lux operon night light | ||
: <b>Discussion Points</b> | : <b>Discussion Points</b> | ||
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::*use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.' | ::*use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.' | ||
:*expression of lux as controlled by lac may not be enough without available lactose | :*expression of lux as controlled by lac may not be enough without available lactose | ||
| - | ::**question: Is lactose membrane permeable for S. PCC6803? | + | ::**question: Is lactose membrane permeable for S. PCC6803? [http://cyano.genome.jp/cgi-bin/cyorf_view.cgi?ORG=syn&ACCESSION=slr1202 YES] PCC6803 encodes two lactose permease proteins, lacF and lacG. |
::**may need to find a membrane permeable substance that can bind to the lac promoter | ::**may need to find a membrane permeable substance that can bind to the lac promoter | ||
Krystle: bacterial export system, soluble proteins | Krystle: bacterial export system, soluble proteins | ||
Revision as of 02:59, 6 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
Agenda
9am St. John 515
- Update progress on experiments performed this week
- competent cell creation (taken 2 days), and testing results
- Part B proposals
- Grace
- Krystle
- Margaret (teleconference in from US mainland)
Minutes
Present: GK, KS, MR, SC, GP, KLS, NW, LG
Presentations:
Grace: lux operon night light
- Discussion Points
- rbc may be too strong a promoter for use in the proposed construct
- test the strength of RBC and explore if it is leaky by creating a lacZ/GFP fusion protein
- use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.'
- expression of lux as controlled by lac may not be enough without available lactose
- question: Is lactose membrane permeable for S. PCC6803? [http://cyano.genome.jp/cgi-bin/cyorf_view.cgi?ORG=syn&ACCESSION=slr1202 YES] PCC6803 encodes two lactose permease proteins, lacF and lacG.
- may need to find a membrane permeable substance that can bind to the lac promoter
Krystle: bacterial export system, soluble proteins
- Discussion Points
- it will be best to focus on pilA and slr2016, signal sequences that have already been experimentally confirmed
Margaret: synthetic plasmid biobricks
- Discussion Points
Action Items
- <Person A>: Task
Coming Up
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
