Team:Hawaii/Meeting/2008-06- 5

From 2008.igem.org

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(New page: {{Team:Hawaii/Header}} == Agenda == 9am St. John 515 # Update progress on experiments performed this week ## competent cell creation (taken 2 days), and testing results # Part B proposals...)
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== Minutes ==
== Minutes ==
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Present: GK, KS, MR, SC, GP, KS, NW, LG
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Presentations:
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Grace: lux operon night light
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: <b>Discussion Points</b>
 +
:*rbc may be too strong a promoter for use in the proposed construct
 +
:*test the strength of RBC and explore if it is leaky by creating a lacZ/GFP fusion protein
 +
::*use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.'
 +
:*expression of lux as controlled by lac may not be enough without available lactose
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::**question: Is lactose membrane permeable for S. PCC6803?  'yes'
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::**may need to find a membrane permeable substance that can bind to the lac promoter
 +
Krystle: bacterial export system, soluble proteins
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: <b>Discussion Points</b>
 +
:*it will be best to focus on pilA and slr2016, signal sequences that have already been experimentally confirmed
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Present: &lt;Person A&gt;, &lt;Person B&gt;
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Margaret: synthetic plasmid biobricks
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: <b>Discussion Points</b>
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#
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== Action Items ==
== Action Items ==

Revision as of 02:48, 6 June 2008

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Contents

Agenda

9am St. John 515

  1. Update progress on experiments performed this week
    1. competent cell creation (taken 2 days), and testing results
  2. Part B proposals
    1. Grace
    2. Krystle
    3. Margaret (teleconference in from US mainland)

Minutes

Present: GK, KS, MR, SC, GP, KS, NW, LG Presentations: Grace: lux operon night light

Discussion Points
  • rbc may be too strong a promoter for use in the proposed construct
  • test the strength of RBC and explore if it is leaky by creating a lacZ/GFP fusion protein
  • use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.'
  • expression of lux as controlled by lac may not be enough without available lactose
    • question: Is lactose membrane permeable for S. PCC6803? 'yes'
    • may need to find a membrane permeable substance that can bind to the lac promoter

Krystle: bacterial export system, soluble proteins

Discussion Points
  • it will be best to focus on pilA and slr2016, signal sequences that have already been experimentally confirmed

Margaret: synthetic plasmid biobricks

Discussion Points

Action Items

  • <Person A>: Task

Coming Up


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