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Team:Hawaii/Milestone
From 2008.igem.org
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* 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated) | * 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated) | ||
* 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR) | * 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR) | ||
| - | * | + | * 2008.06.12 Final draft of proposal due |
| + | * 2008.06.23 Transform PCC6803 w/ pRL1383a | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
Revision as of 04:04, 24 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Immediate
- ASAP: Design and order primers
- 6/12/08 Make list of BioBrick parts needed for Part B
- Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
- Compare RP4 sequence with OriT (MR)
Near Term
- 2008.06.27 Make final decision on Part B of our iGEM project
- RSF1010 (pRL1383a) MCS Replaced
Completed Milestones
- 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
- 2008.05.24 Create seed stocks for test transformation (NW/MR)
- 2008.05.22 Describe, present part types. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
- 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
- 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
- 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
- 2008.06.04 GK and KS are now autoclave trained
- 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
- 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
- 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
- 2008.06.12 Final draft of proposal due
- 2008.06.23 Transform PCC6803 w/ pRL1383a
