Team:Hawaii/Milestone

From 2008.igem.org

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{{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]]
== Immediate ==
== Immediate ==
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<onlyinclude>
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* Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
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* Express GFP constructs in ''Synechocystis''
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</onlyinclude>
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== Near Term ==
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* <strike>OD Reading (AB/NW)</strike>
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== Completed Milestones ==
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* Culturing Protocol Optimization (KS)
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* 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
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* Plasmid Transformation Plan/Protocol (GK/MR)
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* [[Team:Hawaii/Notebook/2008-05-22|2008.05.24]] Create seed stocks for test transformation (NW/MR)
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* Plasmid Transformation (GK/MR)
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* [[Team:Hawaii/Meeting/2008-05-22|2008.05.22]] Describe, present [http://partsregistry.org/Part_Types part types]. 1.  Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
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* 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
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== Long Term ==
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* 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
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* 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
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== Milestones ==
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* 2008.06.04 GK and KS are now autoclave trained
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* 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
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[[Team:Hawaii/Meeting/2008-06-27|2008.06.27]] Make final decision on Part B of our iGEM project
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* 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
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* 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle.  linear DNA need recombination.  Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
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<!-- Team Hawaii Tracker -->
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* 2008.06.12 Final draft of proposal due
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* 2008.06.23 Transform PCC6803 w/ pRL1383a
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<script type="text/javascript">
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* 2008.06.24 First batch of primers ordered
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var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
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* 2008.06.24 Biobrick parts list completed
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document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E"));
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* 2008.06.24 RP1 and RP4 oriT are the same
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</script>
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* 2008.08.13 Replaced pRL1383a MCS with BioBrick MCS (GK)
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<script type="text/javascript">
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{{Team:Hawaii/Footer}}
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var pageTracker = _gat._getTracker("UA-308155-6");
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pageTracker._initData();
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pageTracker._trackPageview();
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</script>
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</html>
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<!-- Team Hawaii Tracker -->
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Latest revision as of 17:35, 15 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)
20080612-work breakdown structure.jpg

Immediate

  • Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
  • Express GFP constructs in Synechocystis

Near Term

Completed Milestones

  • 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
  • 2008.05.24 Create seed stocks for test transformation (NW/MR)
  • 2008.05.22 Describe, present [http://partsregistry.org/Part_Types part types]. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
  • 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
  • 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
  • 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
  • 2008.06.04 GK and KS are now autoclave trained
  • 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
  • 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
  • 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
  • 2008.06.12 Final draft of proposal due
  • 2008.06.23 Transform PCC6803 w/ pRL1383a
  • 2008.06.24 First batch of primers ordered
  • 2008.06.24 Biobrick parts list completed
  • 2008.06.24 RP1 and RP4 oriT are the same
  • 2008.08.13 Replaced pRL1383a MCS with BioBrick MCS (GK)

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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