Team:Hawaii/Milestone

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Revision as of 01:49, 6 June 2008 by Gracek (Talk | contribs)
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Immediate

  • 6/12/08 Final draft of proposal due
  • 6/12/08 Design and order primers
  • 6/12/08 Make list of BioBrick parts needed for Part B
  • Culturing Protocol Optimization (KS)
  • Find fluorometer
  • Does RSF1010 replicate autonomously in PCC6803? (MR)
  • Compare RP4 sequence with OriT (MR)


Long Term

  • 2008.06.27 Make final decision on Part B of our iGEM project
  • Learn how to extract plasmids from BioBrick kit filter paper -> test transform

Completed Milestones

  • 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
  • 2008.05.24 Create seed stocks for test transformation (NW/MR)
  • 2008.05.22 Describe, present [http://partsregistry.org/Part_Types part types]. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
  • 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
  • 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
  • 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
  • 2008.06.04 GK and KS are now autoclave trained


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