Team:Hawaii/Notebook/2008-05-30

From 2008.igem.org

(Difference between revisions)
(Preparation for making competant cells)
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*Made [https://2008.igem.org/SOB SOB media] for growing ''E. coli'' DH5-alpha
*Made [https://2008.igem.org/SOB SOB media] for growing ''E. coli'' DH5-alpha
*Made SOC media
*Made SOC media
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*Made 20mM glucose solution
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*Made 20mM glucose 12.5X stock solution (12.5X 20mM = 0.25M)
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*Made CMB80 media (need to filter in detergent free bottle tomorrow)
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*Made CMB80 media <strike>(need to filter in detergent free bottle tomorrow)</strike> (already filtered into plastic sterile bottle that came with the filtering kit, stored @ 4C)
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*Made stock of 1 M KoAc
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*Made stock of 1 M KOAc
*Made stock of 0.1 N HCl
*Made stock of 0.1 N HCl

Revision as of 07:28, 31 May 2008

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Contents

Plated E. coli

Grace & Norman

Plated E. coli strain DH5-alpha on LB plates made yesterday

  • one plate was plated using the hockey puck method for maximum growth
  • one plate was plated using the 4-way streak method to isolate colonies

Plates were placed in a 37C incubator for 5 hours then transfered to a 4C incubator for storage.

Preparation for making competant cells

Grace, Margaret, & Norman

  • Made SOB media for growing E. coli DH5-alpha
  • Made SOC media
  • Made 20mM glucose 12.5X stock solution (12.5X 20mM = 0.25M)
  • Made CMB80 media (need to filter in detergent free bottle tomorrow) (already filtered into plastic sterile bottle that came with the filtering kit, stored @ 4C)
  • Made stock of 1 M KOAc
  • Made stock of 0.1 N HCl

Discussion

Grace, Margaret, and Norman will come in on Sunday at 3pm to grow up E. coli into exponential phase in preparation for inducing chemical competency.

Quote of the Day

Dr. Presting: "Wow, I didn't know three people could fit in this room."
M: "Well, not maybe three of you."
Dr. Presting: "Are you calling me fat?"
M: "No, no. You're tall, dark, and handsome!"


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