Team:Hawaii/Notebook/2008-06-10

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Things we did today

Wetlab work

Made LB+Kan50 plates

Grace
  • Made LB+Kan50 plates

Preparation for tri-parental conjugation

Grace
  • Streaked out DH5α transformed with pRL1383a on LB+sp for conjugation tomorrow
  • Streaked out E. coli transformed with RP1 on LB+kan for conjugation tomorrow

Biobrick extraction/transformation

Grace
  • Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. (see experiment)
Norman
  • Streaked out transformants from yesterday's BioBricks transformation/extraction

Drylab Work

  • Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO2 incubation
  • Got Anabena triparental conjugation protocol from SC
  • Found [http://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/109 nifty paper] on cyanobacteria proteins/gene location.

Discussion

  • Norman will figure out concentration of DNA on Biobricks filter paper
  • Norman will make LB+amp100 plates tonight
  • Need to check incubated plates tomorrow (Krystle?)
  • Need to create growth curve (OD750 vs. cell population)
  • Need to practice lifting agar for antibiotic insertion (Thursday?)

Quote of the Day

As long as you don’t know what it is, you don’t need to fill out forms.


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