Team:Hawaii/Notebook/2008-06-14

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab Work== ===Cell density test for PCC6803=== :<strong> Krystle</strong> :* Plated 10<sup>-5</sup>, 10<sup>-7</sup>, 10<sup>-7</sup...)
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:<strong> Krystle</strong>
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:* Plated 10<sup>-5</sup>, 10<sup>-7</sup>, 10<sup>-7</sup> dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
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:* Plated 10<sup>-5</sup>, 10<sup>-7</sup>, 10<sup>-8</sup> dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
:* Plates in SC incubator
:* Plates in SC incubator
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= Quote of the Day =
= Quote of the Day =
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<blockquote>''"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS''</blockquote>
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:''"Yeah, I'm trying to stave off the real world by staying in school a little longer."'' - KS
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<br>
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:''"Ooooooooooooooooooooooooh"'' -KS
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:: ''"Yes Krystle, it's shiny."'' -GK
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}
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Latest revision as of 00:56, 15 June 2008

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Things we did today

Wetlab Work

Cell density test for PCC6803

Krystle
  • Plated 10-5, 10-7, 10-8 dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
  • Plates in SC incubator

Glycerol stocked Biobrick colonies

Krystle
  • Combined 0.4ml 80% glycerol w/ 0.8ml of transformed E. coli grown overnight in LB + antibiotic
  • Cryostock could not be made for pUC18 because of contamination in overnight culture

Made competent cells

Grace
  • Same protocol as 6/2 competent cells
  • OD600 = 0.4567 (overnight culture)
  • OD600 = 1.1-1.2 (after CCMB80 added)

Triparental conjugation

Grace
  • Streaked BG-11+sp+sm plates with conjugates
  • Incubated in light & CO2 @ 30C in SC incubator

Discussion

For competent cells, should cool eppendorf tray at 4C. After aliquot, transfer tubes to -80C EtOH tray and freeze at -80C. GP suggests we aliquot with tubes in dry ice.

Was Callahan lab hood working?

Quote of the Day

"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS


"Ooooooooooooooooooooooooh" -KS
"Yes Krystle, it's shiny." -GK

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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