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Team:Hawaii/Notebook/2008-06-14
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab Work== ===Cell density test for PCC6803=== :<strong> Krystle</strong> :* Plated 10<sup>-5</sup>, 10<sup>-7</sup>, 10<sup>-7</sup...) |
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= Quote of the Day = | = Quote of the Day = | ||
<blockquote>''"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS''</blockquote> | <blockquote>''"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS''</blockquote> | ||
| - | + | <blockquote>''Ooooooooooooooooooooooooh'' -KS, ''Yeah Krystle, it's shiny.'' -GK</blockquote> | |
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 23:16, 14 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab Work
Cell density test for PCC6803
- Krystle
- Plated 10-5, 10-7, 10-7 dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
- Plates in SC incubator
Glycerol stocked Biobrick colonies
- Krystle
- Combined 0.4ml 80% glycerol w/ 0.8ml of transformed E. coli grown overnight in LB + antibiotic
- Cryostock could not be made for pUC18 because of contamination in overnight culture
Made competent cells
- Grace
- Same protocol as 6/2 competent cells
- OD600 = 0.4567 (overnight culture)
- OD600 = 1.1-1.2 (after CCMB80 added)
Triparental conjugation
- Grace
- Streaked BG-11+sp+sm plates with conjugates
- Incubated in light & CO2 @ 30C in SC incubator
Discussion
For competent cells, should cool eppendorf tray at 4C. After aliquot, transfer tubes to -80C EtOH tray and freeze at -80C. GP suggests we aliquot with tubes in dry ice.
Was Callahan lab hood working?
Quote of the Day
"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS
Ooooooooooooooooooooooooh -KS, Yeah Krystle, it's shiny. -GK
