Team:Hawaii/Notebook/2008-06-14

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Things we did today

Wetlab Work

Cell density test for PCC6803

Krystle
  • Plated 10-5, 10-7, 10-7 dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
  • Plates in SC incubator

Glycerol stocked Biobrick colonies

Krystle
  • Combined 0.4ml 80% glycerol w/ 0.8ml of transformed E. coli grown overnight in LB + antibiotic
  • Cryostock could not be made for pUC18 because of contamination in overnight culture

Made competent cells

Grace
  • Same protocol as 6/2 competent cells
  • OD600 = 0.4567 (overnight culture)
  • OD600 = 1.1-1.2 (after CCMB80 added)

Triparental conjugation

Grace
  • Streaked BG-11+sp+sm plates with conjugates
  • Incubated in light & CO2 @ 30C in SC incubator

Discussion

For competent cells, should cool eppendorf tray at 4C. After aliquot, transfer tubes to -80C EtOH tray and freeze at -80C. GP suggests we aliquot with tubes in dry ice.

Was Callahan lab hood working?

Quote of the Day

"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS


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