Team:Hawaii/Notebook/2008-06-18

From 2008.igem.org

(Difference between revisions)
(Drylab Work)
(Wetlab work)
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:'''KS'''
:'''KS'''
''cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify''
''cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify''
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
 
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:* e.g. worked on <blah experiment link>, PCR, ran gel
 
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=== Counted transformation efficiency of new competent cells===
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: '''Grace'''
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{| border="1"
 +
|+ '''Colony Count of Transformants'''
 +
!Plasmid
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!Dilution
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!Colony Forming Units on Plate
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!Number of Transformed Cells per mL
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|-
 +
| rowspan="3" align="center"|pBluescript||align="center"| 1:1 || align="center"|79|| align="center"|3950
 +
|-
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|  align="center"|1:10|| align="center"|12|| align="center"|6000
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|-
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|  align="center"|1:100|| align="center"|1||align="center"|5000
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|-
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| " align="center"|BBa_B0024||align="center"|1:1||align="center"|0||align="center"|n/a
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|-
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| align="center"|BBa_B0025||align="center"|1:1|| align="center"|1|| align="center"|50
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|-
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| align="center"|BBa_E0240||align="center"|1:1|| align="center"|0 ||align="center"|n/a
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|}
= Discussion =
= Discussion =

Revision as of 17:24, 19 June 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Test Transform pRL1383a Plasmids from yesterday

KS

cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify

Counted transformation efficiency of new competent cells

Grace
Colony Count of Transformants
Plasmid Dilution Colony Forming Units on Plate Number of Transformed Cells per mL
pBluescript 1:1 793950
1:10126000
1:10015000
BBa_B00241:10n/a
BBa_B00251:1150
BBa_E02401:10 n/a

Discussion

Quote of the Day

"What's that band-aid for on your hand"-NW

"I was testing out this bottle of anesthetics then pricked myself with needles"-KS
"Umm OK..."-NW


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]