Team:Hawaii/Notebook/2008-06-18

From 2008.igem.org

(Difference between revisions)
(Wetlab work)
(Counted transformation efficiency of new competent cells)
Line 16: Line 16:
!Number of Transformed Cells per mL
!Number of Transformed Cells per mL
|-
|-
-
| rowspan="3" align="center"|pBluescript||align="center"| 1:1 || align="center"|79|| align="center"|3950
+
| rowspan="3" align="center"|pUC18||align="center"| 1:1 || align="center"|79|| align="center"|3950
|-
|-
|  align="center"|1:10|| align="center"|12|| align="center"|6000
|  align="center"|1:10|| align="center"|12|| align="center"|6000

Revision as of 17:24, 19 June 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Test Transform pRL1383a Plasmids from yesterday

KS

cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify

Counted transformation efficiency of new competent cells

Grace
Colony Count of Transformants
Plasmid Dilution Colony Forming Units on Plate Number of Transformed Cells per mL
pUC18 1:1 793950
1:10126000
1:10015000
BBa_B00241:10n/a
BBa_B00251:1150
BBa_E02401:10 n/a

Discussion

Quote of the Day

"What's that band-aid for on your hand"-NW

"I was testing out this bottle of anesthetics then pricked myself with needles"-KS
"Umm OK..."-NW


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]