Team:Hawaii/Notebook/2008-06-18

From 2008.igem.org

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Test Transform pRL1383a Plasmids from yesterday

KS

cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify

Counted transformation efficiency of new competent cells

Grace
Colony Count of Transformants
Plasmid Dilution Colony Forming Units on Plate Number of Transformed Cells per mL
pUC18 1:1 793950
1:10126000
1:10015000
BBa_B00241:10n/a
BBa_B00251:1150
BBa_E02401:10 n/a

Discussion

Even though our cells appear more competent than the last batch, we still have a long way to go compared to the commercial grade competent cells. It would be a better idea to chill the tubes, aliquot, then ethanol freeze them.

Quote of the Day

"What's that band-aid for on your hand"-NW

"I was testing out this bottle of anesthetics then pricked myself with needles"-KS
"Umm OK..."-NW


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]