Team:Hawaii/Notebook/2008-06-24

From 2008.igem.org

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep for pRL1383a=== :<strong> Margaret & Grace</strong> :* Grew up transformed ''E. coli'' and did a plasmid ...)
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:* Ordered primers
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:* Doublecheck primers, corrected errors.
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===Grace===
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:* Ordered primers (total 38 oligos)
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===Wiki===
:<strong>Grace</strong>
:<strong>Grace</strong>
:* Updated protocols section of wiki with all successfully tested protocols
:* Updated protocols section of wiki with all successfully tested protocols
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= Discussion =
= Discussion =

Latest revision as of 07:44, 25 June 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep for pRL1383a

Margaret & Grace
  • Grew up transformed E. coli and did a plasmid prep to isolate pRL1383a
  • Made RNase A stock solution (10 mg/ml)
  • 1 μl RNase A added alongside GTE solution
  • 1M Tris-HCl (pH 7.5) also made

Made LB liquid media

Grace


Drylab Work

Primers

Norman
  • Doublecheck primers, corrected errors.
  • Ordered primers (total 38 oligos)

Wiki

Grace
  • Updated protocols section of wiki with all successfully tested protocols

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]