Team:Hawaii/Notebook/2008-06-24

From 2008.igem.org

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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep for pRL1383a

Margaret & Grace
  • Grew up transformed E. coli and did a plasmid prep to isolate pRL1383a
  • Made RNase A stock solution (10 mg/ml)
  • 1 μl RNase A added alongside GTE solution
  • 1M Tris-HCl (pH 7.5) also made

Made LB liquid media

Grace


Drylab Work

Primers

Norman
  • Doublecheck primers, corrected errors.
  • Ordered primers (total 38 oligos)

Wiki

Grace
  • Updated protocols section of wiki with all successfully tested protocols

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]