Team:Hawaii/Notebook/2008-06- 4

From 2008.igem.org

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Contents

Things we did today

Wetlab work

Make Kan50 1000x Concentrate Stock

Krystle
  • Nanopure H2O + Kanamycin
  • stored in -20C freezer -> antibiotics box

Make Amp50, Amp50+Kan20 LB Plates

Krystle & Norman
  • made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media here]
    • x10 Amp 50ug/mL
    • x10 Amp 50ug/mL + Kan 20ug/mL plates
  • left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.

Drylab Work

Signal Peptide Research

Krystle
  • found two papers with leader sequences {please post on CiteULike}
Norman
  • blast sequence on Cu chealating protein, found PCC6803 gene and used [http://www.cbs.dtu.dk/services/SignalP/ SignalP 3.0] for signal peptide prediction

Discussion on CO2 Incubator Controller

Dr. Gautz, Krystle & Norman
  • KS will do some fact finding about the parts required
  • KS will put together schematic for CO2 regulator design

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]