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Team:Hawaii/Notebook/2008-07-29
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Gel purification of ccdB PCR product=== :<strong>Grace</strong> ===RE digests=== :<strong> Grace</strong> :* Digested...) |
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===Transformation of constructs in DB3.1=== | ===Transformation of constructs in DB3.1=== | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
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| + | ===Direct Band Extraction Method=== | ||
| + | :* make two comb gels | ||
| + | :* run test UPA band along with ladder | ||
| + | :* attempt to pipette out DNA as it runs into the second well | ||
| + | :* no more gel cutting??? | ||
== Drylab Work == | == Drylab Work == | ||
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= Quote of the Day = | = Quote of the Day = | ||
| - | + | LOL! http://www.youtube.com/watch?v=J0s0Y3-BCaw | |
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| - | + | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 03:18, 30 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Gel purification of ccdB PCR product
- Grace
RE digests
- Grace
- Digested pRL1383a (from last night) with BamHI
- Digested B0030 and B0024 (from last night) with EcoRI
- Sequentially digested gel purified ccdB with HindIII and BamHI
Construction of BioBricks
- Grace
- Purified RE digests on EtBr stained 3% agarose gel; extracted desired bands
- Ligated pRL1383a with ccdB, nir with B0030, and GFP and GFPf with B0024
Transformation of constructs in DB3.1
- Grace
Direct Band Extraction Method
- make two comb gels
- run test UPA band along with ladder
- attempt to pipette out DNA as it runs into the second well
- no more gel cutting???
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
LOL! http://www.youtube.com/watch?v=J0s0Y3-BCaw
