Team:Hawaii/Notebook/2008-07-29

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Things we did today

Wetlab work

Gel purification of ccdB PCR product

Grace

RE digests

Grace
  • Digested pRL1383a (from last night) with BamHI
  • Digested B0030 and B0024 (from last night) with EcoRI
  • Sequentially digested gel purified ccdB with HindIII and BamHI

Construction of BioBricks

Grace
  • Purified RE digests on EtBr stained 3% agarose gel; extracted desired bands
  • Ligated pRL1383a with ccdB, nir with B0030, and GFP and GFPf with B0024

Transformation of constructs in DB3.1

Grace

Direct Band Extraction Method

  • make two comb gels
  • run test UPA band along with ladder
  • attempt to pipette out DNA as it runs into the second well
  • no more gel cutting???

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

LOL! http://www.youtube.com/watch?v=J0s0Y3-BCaw