Team:Hawaii/Notebook/2008-07- 9

From 2008.igem.org

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:::: pilA, slr1, slr2 combined with homemade competent cells (batch 3)
:::: pilA, slr1, slr2 combined with homemade competent cells (batch 3)
:::: pnir combined with commercial supercompetent cells.
:::: pnir combined with commercial supercompetent cells.
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===Streak Out DB3.1===
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:'''Krystle'''
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:* colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
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:* DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
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:* Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)
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[[Image:
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===Run PCR Products on Gel===
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:'''Margaret'''
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:* PCR amplification of Omega cassette and OriR
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:* see [[Team:Hawaii/PCR_Amplification_of_pRL1383a| here for updates for the pRL1383a amplification]]
== Drylab Work ==
== Drylab Work ==

Latest revision as of 03:41, 15 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Transformation of DH5a with synthetic oligonucleotide ligations

Krystle
  • Followed protocol used for transformation of cells with BioBrick plasmids from filter
pilA, slr1, slr2 combined with homemade competent cells (batch 3)
pnir combined with commercial supercompetent cells.

Streak Out DB3.1

Krystle
  • colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
  • DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
  • Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)

[[Image:

Run PCR Products on Gel

Margaret

Drylab Work

Wiki updates

Grace
  • Updated "Protocols" section with new protocols.
  • Updated Initial Oligo Synthesis experiment

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]