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Team:Hawaii/Notebook/2008-09-12
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colony forming units |
|---|---|
| 3A: nir+rbs + slr1 | 4 |
| 3A: nir+ rbs + pilA | 32 |
| 3A: nir+rbs + slr1+GFPf | 35 |
| 3A: nir+rbs + pilA+GFPf (8/12) | 42 |
| 3A: nir+rbs + pilA+GFPf (8/13) | 24 |
| pSB1A3 (-) | 25 |
| plac+rbs + slr1 | >300 |
| plac+rbs + pilA | >300 |
| plac+rbs + slr1+GFPf | >300 |
| plac+rbs + pilA+GFPf (8/12) | >300 |
| plac+rbs + pilA+GFPf (8/13) | >300 |
| plac+rbs (-) | 295 |
| nir+rbs + slr1 | >300 |
| nir+rbs + pilA | >300 |
| nir+rbs + slr1+GFPf | >300 |
| nir+rbs + pilA+GFPf (8/12) | >300 |
| nir+rbs + pilA+GFPf (8/13) | >300 |
| nir+rbs (-) | 301 |
- Colony PCR of 3A, plac+rbs transformants
Made competent DH5α cells
- Grace & Margaret
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
