Team:Hawaii/Notebook/2008-09-12

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Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.
Construct Colony forming units
3A: nir+rbs + slr1 4
3A: nir+ rbs + pilA 32
3A: nir+rbs + slr1+GFPf 35
3A: nir+rbs + pilA+GFPf (8/12) 42
3A: nir+rbs + pilA+GFPf (8/13) 24
pSB1A3 (-) 25
plac+rbs + slr1 >300
plac+rbs + pilA >300
plac+rbs + slr1+GFPf >300
plac+rbs + pilA+GFPf (8/12) >300
plac+rbs + pilA+GFPf (8/13) >300
plac+rbs (-) 295
nir+rbs + slr1 >300
nir+rbs + pilA >300
nir+rbs + slr1+GFPf >300
nir+rbs + pilA+GFPf (8/12) >300
nir+rbs + pilA+GFPf (8/13) >300
nir+rbs (-) 301
  • Colony PCR of 3A, plac+rbs transformants



Discussion

Quote of the Day

Wait, you have an internet boyfriend? - KS, to Norman


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