"
Team:Hawaii/Notebook/2008-09-19
From 2008.igem.org
(Difference between revisions)
(→Construction of secretion device) |
(marg) |
||
| Line 22: | Line 22: | ||
::* EcoRI/PstI | ::* EcoRI/PstI | ||
:::* pSB1A3 | :::* pSB1A3 | ||
| + | |||
| + | ===Gel to confirm transformants=== | ||
| + | <strong>Margaret</strong> | ||
| + | :* | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 03:56, 20 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep of BB-pRL1383a
- Grace
Construction of secretion device
- Grace
- PCR of nir+rbs, rbs+GFP, slr1+GFPf, pilA, slr2
- Overnight RE digest:
- EcoRI/SpeI
- nir+rbs
- rbs+GFP
- slr1+GFPf
- nir (PCR product from before)
- XbaI/PstI
- rbs+GFP
- slr1+GFPf
- pilA
- slr2
- EcoRI/PstI
- pSB1A3
Gel to confirm transformants
Margaret
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
