Team:Hawaii/Notebook/2008-09-19

From 2008.igem.org

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Things we did today

Wetlab work

Plasmid prep of BB-pRL1383a

Grace

Construction of secretion device

Grace
  • PCR of nir+rbs, rbs+GFP, slr1+GFPf, pilA, slr2
  • Overnight RE digest:
  • EcoRI/SpeI
  • nir+rbs
  • rbs+GFP
  • slr1+GFPf
  • nir (PCR product from before)
  • XbaI/PstI
  • rbs+GFP
  • slr1+GFPf
  • pilA
  • slr2
  • EcoRI/PstI
  • pSB1A3
Krystle
  • Transformation of overnight ligations

Gel to confirm transformants

Margaret


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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