Team:Hawaii/Notebook/2008-09-23

From 2008.igem.org

(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* pSB1A3 :* rbs+GFPf+tt #1, 2 :* nir+rbs+GFP #1, 4, 7 :* nir+rbs+slr1+GFPf #...)
(margaret)
Line 11: Line 11:
:* nir+rbs+slr1+GFPf #2, 8
:* nir+rbs+slr1+GFPf #2, 8
:* nir+rbs+pilA #18
:* nir+rbs+pilA #18
 +
 +
===Sequencing===
 +
:<strong> Margaret</strong>
 +
[[Image:rep_9_23.jpg|right|thumb|300px|1st lane is ladder, 2nd lane is rep amplified from a colony, 3rd lane is rep amplified from a restriction product of rep from the same colony.]]
 +
 +
:*PCR of rep, ran a gel to verify size, and exo-sap. will send in tomorrow

Revision as of 03:31, 24 September 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep

Grace
  • pSB1A3
  • rbs+GFPf+tt #1, 2
  • nir+rbs+GFP #1, 4, 7
  • nir+rbs+slr1+GFPf #2, 8
  • nir+rbs+pilA #18

Sequencing

Margaret
1st lane is ladder, 2nd lane is rep amplified from a colony, 3rd lane is rep amplified from a restriction product of rep from the same colony.
  • PCR of rep, ran a gel to verify size, and exo-sap. will send in tomorrow


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]